Thermo ScientificTM PhireTM Animal Tissue Direct PCR Kit is designed for DNA amplification directly from animal tissues. Here we present protocols for transgenic mice genotyping achieved directly from mouse ear tissues usi...
The double transgene did not appear to cause embryonic lethality, as the mice were born at Mendellian ratios and were healthy for the first month of life. Genotyping primers are listed (Supplementary Table S12). Animal stud- ies were approved by the National Cancer Institute (NCI) ...
animal purchase, animal care, hormone treatment, superovulation, oocyte harvest, DNA microinjection, embryo culture, embryo transfer, near natal care, sampling of mouse and rabbit kids, transgene PCR genotyping, and original generation of mouse and rabbit breeding...
Genotype pups by PCR to identify those carrying the transgene. Types of Transgenic Mice Services Preparation of DNA for injection and development of a genotyping strategy The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ...
Genotyping was confirmed by PCR and performed at 3 weeks of age to determine study groups. Mixed gender F1 mouse generation was used in this study. Animals were housed in polycarbonate cages (15 × 23 × 17 cm) provided with a mouse house and aspen bedding and ...
PiggyBAC transgenic mice provide high and consistent expression of a transgene, improving model data reliability for research of human diseases and drug treatments. Our new PiggyBac transgenic mouse service can deliver single-copy transgenic mice with m
Universal PCR Genotyping Assay that Achieves Single copy Sensitivity with Any Primer Pair. Transgenic Res 12, 521–522 (2003). https://doi.org/10.1023/A:1024225408961 Download citation Issue DateAugust 2003 DOIhttps://doi.org/10.1023/A:1024225408961 genotype knockout mouse PCR transgenic Access ...
An unbalanced increase in dietary omega-6 (n-6) polyunsaturated fatty acids (PUFA) and decrease in omega-3 (n-3) PUFA in the Western diet coincides with the global rise in chronic diseases. Whether n-6 and n-3 PUFA oppositely contribute to the developmen
Genotyping was performed by PCR and resulting products were analyzed by electrophoresis on 1.5% agarose gels. Oocyte isolation and culture Fully-grown GV-intact oocytes were obtained from eight-week old mice 44 hours after superovulation by intraperitoneal injection of 0.1 ml (5 units) of PMSG (...
A total of 124 parvalbumin (PV)-Cre mice were generous gifts from Prof. You Wan (School of Medicine, Tsinghua University, Beijing, China). Mouse genotyping was performed following protocols described in the relevant literature [22,23,24]. P301L tau transgenic pR5 mice were generated on a ...