Development and use of TCR transgenic mice for malaria immunology research. Methods Molecular Biology 923, 481-491 (2013).CHEN, Y. C. & ZAVALA, F. 2013. Development and use of TCR transgenic mice for malaria immunology research. Methods Mol Biol, 923, 481-91....
T cells react to foreign or self-antigens through T cell receptor (TCR) signaling. Several decades of research have delineated the mechanism of TCR signal transduction and its impact on T cell performance. This knowledge provides the foundation for chimeric antigen receptor T cell (CAR-T cell)...
Next, we used parabiosis to formally test whether the endogenously expanded SPAS-1+ T cells that accumulated in NLTs were tissue-resident. This surgical approach joins the vasculatures of two mice and allows distinction between cells that can freely circulate between the parabiont partners and cell...
We have constructed mice containing the human Vβ3 TCR gene from the influenza virus haemagglutinin specific human CD4+ T cell clone HA1.7. Similar cell yields were obtained from transgenic and non-transgenic lymphoid tissue, with normal levels of T cells and with no unusual bias of the CD4 ...
It has been widely assumed that T cells from TCR-transgenic (Tg) mice better represent the behavior of T cells from normal mice than do in vitro cultures of T cell clones. We have found that autoreactive T cells arising in the presumably more physiological environment of the BDC-2.5 TCR-...
It is also demonstrated in this study that H-Y/Db-specific CD8+ T cells from H-Y TCR transgenic mice were unable to lyse target cells expressing H-Y antigen. Stimulation of H-Y-specific effector CD8+ T cells with H-Y antigen, although insufficient to induce lytic granule release, was ...
DO11.10 mice are Tg for an αβ TCR which recognizes both an OVA-derived peptide and the superantigen, SEB (Murphy et al., Science, 250: 1720, 1990). Flow cytometric analyses indicated that >90% of the CD4+ T cells were Vβ8+ but only 50-60% were clonotype+ (KJ1-26), indicating...
TCR-T: T cell receptor-engineered T cells TCRs: T cell receptors TME: Tumor microenvironment TNF: Tumor necrosis factor TRAIL: Tumor necrosis factor-related apoptosis-inducing ligand TAAs: Tumor-associated antigens TIL: Tumor-infiltrating lymphocytes ...
It is thought that Tcrbeta genes are effectively allelically excluded, while Tcralpha genes are not. We report here that endogenous Tcrbeta genes are expressed on as much as 5-7% of CD4+ T cells in 8-week-old Tcralphabeta-transgenic mice. In this model, the transgenic Tcr recognizes resi...
However, SCA-1 levels were increased in CD8+ Tn cells in CH mice regardless of the Ly6C expression. Another surface marker, CD5 was highly expressed on Ly6C+ cells from both SPF and CH mice (Fig. 4E). CD5 is a negative regulator of T cell receptor (TCR)-mediated signaling19 and its...