To investigate the possibility of simultaneously delivering protein expression and gene silencing, we tested whether a taRNA system can carry and release functional miRNA to target cells. Here, we show that mature miRNA is released from STRs and silences specific targets in a replication-dependent ...
In saRNA-based vaccines, replicase and antigens are encoded on the same mRNA molecule, resulting in very long RNA sequences, which poses significant challenges in production, delivery, and stability. In taRNA-based vaccines, these challenges can be overcome by splitting the replication system into...
The final RNA sequence was used to design a trans‐amplifying RNA (taRNA) vaccine in a split‐vector system. It consists of two molecules: a nonreplicating RNA encoding a trans‐acting replicase to amplify the second one, a trans‐replicon (TR) RNA encoding the vaccine ...