CRISPR/Cas9技术是一种基因编辑技术,它是继ZFN、TALENs等基因编辑技术推出后的第三代技术。这种技术依赖于一种由RNA指导的Cas9核酸酶对靶向基因进行特定DNA修饰,能够在生物基因组特定位置进行精确插入、删除、替换及修饰DNA。 CRISPR/Cas9原本是细菌和古细菌为应对病毒和质粒不断攻击而演化来的获得性免疫防御机制。在这个...
Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction is essential for pre-crRNA processing as well as target recognition and ...
trans activating CRICPRRNA 反式激活cricprrna --- 希望能够帮到您！满意请采纳，谢谢~
...NA序列上,包 括启动子和增强子的调控区; 2.反式激活结构域(trans-activating domain): 其通过蛋白与蛋白之间相互作 … www.docin.com|基于 1 个网页 3. 反式激活域 ...n) 以外,还有一个可以激活基因转录的结构域,这一反式激活域(trans-activating domain)使得该转录因子能与结合在RNA 聚… ...
Define transactivating. transactivating synonyms, transactivating pronunciation, transactivating translation, English dictionary definition of transactivating. tr.v. trans·ac·ti·vat·ed , trans·ac·ti·vat·ing , trans·ac·ti·vates To stimulate the
关键词: Humans Deltaretrovirus Chromosome Deletion Transcription Factors Viral Proteins RNA, Viral Chromosome Mapping Base Sequence Repetitive Sequences, Nucleic Acid Gene Expression Regulation DOI: 10.1126/science.2990041 被引量: 1216 年份: 1985
or on other cells in the corpus callosum, which was consistent with RNA-seq data for OPCs28,56. Neither did we detect Nrp2 on microglia, also consistent with the transcriptomic studies28,39,40. Furthermore, the dramatic decrease in OPC proliferation in demyelinated lesions after Nrp1 deleti...
(g) MDA-MB-231 cells were co-transfected with shRNA vector (sh Control or shAURKA) and the overexpression vector (ER or AER) via lentivirus-mediated gene transfer. Puromycin (1 mg ml À 1) and blasticidin (2 mg ml À 1) double selected cells were subjected to CD24/CD44 staining ...
In addition, we detected two HOXB7 transcripts in several breast cell lines and demonstrated that both normal and mutated alleles were expressed at the RNA level in MCF7 cells as well as in a variety of breast tissues and lymphocytes, suggesting that a truncated HOXB7 protein might be ...
RNA was isolated for RT-PCR or cells were fixed for chromatin immunoprecipitation (ChIP) analysis. A. RT-PCR was performed using primers specific for Myf5 and GAPDH. B. ChIP assays were performed using antibodies specific for FLAG or HA or normal mouse IgG. Immunoprecipitated chromatin was ...