CRISPR/Cas9技术是一种基因编辑技术,它是继ZFN、TALENs等基因编辑技术推出后的第三代技术。这种技术依赖于一种由RNA指导的Cas9核酸酶对靶向基因进行特定DNA修饰,能够在生物基因组特定位置进行精确插入、删除、替换及修饰DNA。 CRISPR/Cas9原本是细菌和古细菌为应对病毒和质粒不断攻击而演化来的获得性免疫防御机制。在这个...
Trans-Activating crRNA is a type of guide RNA that forms a binary complex with Cas9 to target specific sequences in double-stranded DNA for genome editing. AI generated definition based on: Methods in Enzymology, 2015 About this pageSet alert ...
trans activating CRICPRRNA 反式激活cricprrna --- 希望能够帮到您！满意请采纳，谢谢~
Define transactivating. transactivating synonyms, transactivating pronunciation, transactivating translation, English dictionary definition of transactivating. tr.v. trans·ac·ti·vat·ed , trans·ac·ti·vat·ing , trans·ac·ti·vates To stimulate the
Both the cells deficient in p63 gene expression by RNA interference and cells that overexpressed the N-terminally truncated p63 isoform α (ΔNp63α), a dominant-negative variant of TAp63α, by gene transfection, survived TLR3 activation. Taken together, TAp63α is a crucial regulator ...
or on other cells in the corpus callosum, which was consistent with RNA-seq data for OPCs28,56. Neither did we detect Nrp2 on microglia, also consistent with the transcriptomic studies28,39,40. Furthermore, the dramatic decrease in OPC proliferation in demyelinated lesions after Nrp1 deleti...
While RNA derived from the ME180 cells yielded a positive signal in the RT-PCR reaction designed to amplify transcripts encoding the acidic N terminus, the primary keratinocytes and the ectocervical cells showed little or no product (Figure 5D, TA). In contrast, RNA from all three sources ...
...NA序列上,包 括启动子和增强子的调控区; 2.反式激活结构域(trans-activating domain): 其通过蛋白与蛋白之间相互作 … www.docin.com|基于 1 个网页 3. 反式激活域 ...n) 以外,还有一个可以激活基因转录的结构域,这一反式激活域(trans-activating domain)使得该转录因子能与结合在RNA 聚… ...
Levin AA. Treating disease at the RNA level with oligonucleotides. N Engl J Med. 2019;380:57–70. PubMed Google Scholar Overman J, Fontaine F, Moustaqil M, Mittal D, Sierecki E, Sacilotto N, et al. Pharmacological targeting of the transcription factor SOX18 delays breast cancer in mice...
Long noncoding RNA-LET, which is repressed by EZH2, inhibits cell proliferation and induces apoptosis of nasopharyngeal carcinoma cell. Med Oncol. 2015;32(9):226. 5. Kooistra SM, Helin K. Molecular mechanisms and potential functions of histone demethylases. Nat Rev Mol Cell Biol. 2012;13(5...