这种技术依赖于一种由RNA指导的Cas9核酸酶对靶向基因进行特定DNA修饰,能够在生物基因组特定位置进行精确插入、删除、替换及修饰DNA。 CRISPR/Cas9原本是细菌和古细菌为应对病毒和质粒不断攻击而演化来的获得性免疫防御机制。在这个系统中,crRNA(CRISPR-derived RNA)通过碱基配对与tracrRNA(trans-activating RNA)结合形成双...
Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction is essential for pre-crRNA processing as well as target recognition and ...
trans activating CRICPRRNA 反式激活cricprrna --- 希望能够帮到您！满意请采纳，谢谢~
Almost any conceivable functional RNA sequence can be overexpressed using this system with occasional sequence modifications. For instance, fluorogenic RNAs are RNA aptamers that bind an otherwise nonfluorescent dye, activating its fluorescence. The field of genetically encodable fluorogenic RNAs began with...
(redirected fromtransactivating) Medical trans·ac·ti·vate (trăns-ăk′tə-vāt′, trănz-) tr.v.trans·ac·ti·vat·ed,trans·ac·ti·vat·ing,trans·ac·ti·vates To stimulate the transcription of (a gene in a host cell) by binding to DNA. Genes can be transactivated natura...
Evidence for a strong transactivating role for RNF12 in Xist activation is provided by many different experimental approaches. If other genes within the Xist TAD have transactivating activity, such an activity is weak, compared to the effect exerted by RNF12. No detectable trans-acting activity ...
...NA序列上,包 括启动子和增强子的调控区; 2.反式激活结构域(trans-activating domain): 其通过蛋白与蛋白之间相互作 … www.docin.com|基于 1 个网页 3. 反式激活域 ...n) 以外,还有一个可以激活基因转录的结构域,这一反式激活域(trans-activating domain)使得该转录因子能与结合在RNA 聚… ...
These results indicate that the HIV-1 rev gene product induces HIV-1 structural gene expression by activating the sequence-specific nuclear export of incompletely spliced HIV-1 RNA species. 展开 关键词: Cell Nucleus Cytoplasm Viral Proteins Gene Products, rev RNA, Viral Chromosome Mapping ...
trans-activation domain 专业医学词典 反式激活功能域 与"trans-activation domain"相近的词条 ... trans-acting ribozyme trans-acting RNA trans-acting transcriptional gene trans-activating response trans-activation trans-activation domain trans-activation property trans-activation response element trans-activator...
B. Computational design of small transcription activating RNAs for versatile and dynamic gene regulation. Nat. Commun. 8, 1051 (2017). Article PubMed PubMed Central Google Scholar Win, M. N. & Smolke, C. D. Higher-order cellular information processing with synthetic RNA devices. Science 322...