这种技术依赖于一种由RNA指导的Cas9核酸酶对靶向基因进行特定DNA修饰,能够在生物基因组特定位置进行精确插入、删除、替换及修饰DNA。 CRISPR/Cas9原本是细菌和古细菌为应对病毒和质粒不断攻击而演化来的获得性免疫防御机制。在这个系统中,crRNA(CRISPR-derived RNA)通过碱基配对与tracrRNA(trans-activating RNA)结合形成双...
Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction is essential for pre-crRNA processing as well as target recognition and ...
Define transactivating. transactivating synonyms, transactivating pronunciation, transactivating translation, English dictionary definition of transactivating. tr.v. trans·ac·ti·vat·ed , trans·ac·ti·vat·ing , trans·ac·ti·vates To stimulate the