pre-crRNAs are processed with the help of atrans-activating crRNA(tracrRNA), leading to the formation of a tracrRNA:crRNA:Cas9 complex that creates double stranded breaks at the target loci[4]. In genome-engineering applications, this tracrRNA-crRNA complex has been replaced by a chimeric single...
CRISPR/Cas9原本是细菌和古细菌为应对病毒和质粒不断攻击而演化来的获得性免疫防御机制。在这个系统中,crRNA(CRISPR-derived RNA)通过碱基配对与tracrRNA(trans-activating RNA)结合形成双链RNA,这个tracrRNA/crRNA二元复合体指导Cas9蛋白在crRNA引导序列靶定位点切断双链DNA。 CRISPR/Cas9技术具有操作简便、效率高、适用性...
Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction is essential for pre-crRNA processing as well as target recognition and ...
Twitter Google Share on Facebook (redirected fromtransactivating) Medical trans·ac·ti·vate (trăns-ăk′tə-vāt′, trănz-) tr.v.trans·ac·ti·vat·ed,trans·ac·ti·vat·ing,trans·ac·ti·vates To stimulate the transcription of (a gene in a host cell) by binding to DNA....
Based on the crystal structure of LbCas12a-crRNA-dsDNA (PDB ID: 5XUS)12, we reasoned that crRNA extensions can influence the trans-cleavage activity by either activating or inhibiting the catalytic efficiency of Cas12a, allowing us to better understand crRNA design with tunable trans-cleavage act...
trans fat, trans-fatn(hydrogenated vegetable oil)SCSimplified Chinese转化型脂肪酸zhuǎn huà xíng zhī fáng suān Your body has no natural mechanism for eliminating trans-fat. trans mann(transgender man)SCSimplified Chinese男变性人 TCTraditional Chinese男變性人 ...
B. Computational design of small transcription activating RNAs for versatile and dynamic gene regulation. Nat. Commun. 8, 1051 (2017). Article PubMed PubMed Central Google Scholar Win, M. N. & Smolke, C. D. Higher-order cellular information processing with synthetic RNA devices. Science 322...
A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously ...
A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously ...
A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously ...