Genomic DNA was obtained from CBD (n= 88), beryllium sensitized (BeS, n= 86), and beryllium exposed nondiseased control subjects (Be-exp, n= 173). Six BTNL2 polymorphisms, HLA-DPB1, DRB1, and DQB1 alleles were determined by sequence-specific primer-PCR. All BTNL2 polymorphisms were in...
The primer pairs F1/R1 and F2/R2 were designed to detect the presence of the loxP site, and F3 and R3 were designed to amplify the DNA fragments spanning the left and right loxPs and the floxed target region. We used genomic DNA for in vitro Cre-loxP-mediated recombination to examine ...
QuantiTect primer assays (Qiagen) for: human ACVR2A (encoding ACTR-IIA), ALK2, ALK3, ALK6, BMP2, BMP4, BMP6, BMP7, BMP9, IL8, NOTCH1, NOTCH2; mouse Bmp2, Bmp6; and rat Bmpr2, Notch1, Notch2, Notch3 and Tnf Reactions were amplified on a StepOnePlus Real-Time PCR system ...
First-strand cDNA was synthesized from half of the DNase-treated GFP mRNA with SuperScript III and a primer specific to the 3′ UTR (MPRA_v3_Amp2Sc_R, Supplementary Data11) using the manufacturer’s recommended protocol, modifying the total reaction volume to 40 μL and performing the elon...
Genotyping was performed using polymerase chain reaction with sequence-specific primers for 2 TNFA promoter variants (rs1800629 and rs361525), 1 variant in the promoter region of IL1A (rs1800587), 2 SNPs in the IL1B gene (rs16944 and rs1 143634), 1 variant in the IL1 receptor (rs...
Primers amplifying rhodopsin, AIF1, GFAP and the retinal ganglion cell marker Pou4F3 [2], [4], [6], [8] were used. cDNA synthesized from the whole retinal RNA was used as a control [1], [3], [5], [7]. 1&2 – rhodopsin, 3&4 – AIF1, 5&6 – GFAP and 7&8 - Pou4...
In this study, we set up a nested polymerase chain reaction-sequence-specific primer (PCR-SSP) approach to type for these TNFa sequence variations. First, sequencing analysis of workshop B lymphoblastoid cell lines ( n =13) showed the presence of three sequence variations upstream of the ...
TNFa-e and HLA-DRB1/DQB1 alleles were identified using sets of sequence-specific primers. Compared to controls, the DRB1*03 and DQB1*02 allele groups, TNFa1 allele, and the TNFa4-b5-c1-d4-e3 and TNFa10-b5-c1-d4-e3 haplotypes were overrepresented in patients. TNF mi...
A 0.9 kb fragment containing sequences homologous with the probe was sequenced and a (GT)9 microsatellite was identified. Further DNA sequencing for the subclones yielded a (TA)10(TTA)5 microsatellite. Primers were designed to amplify these 2 microsatellites in 4 cattle breeds. The PCR product...
Briefly, genomic DNA isolated from test samples were mixed with qRT-PCR master mix and plated on provided qRT- PCR plate containing a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes. Resultant Ct values obtained from RT-PCR were used to represent mtDNA ...