正向引物primer-f:cagcaatgggtaggaga; 反向引物primer-r:cttctcagggccccag。 一种用于检测tnf-a基因snp位点rs1799724基因型的试剂盒,所述的试剂盒包括有上述的引物对和特异探针,所述的特异探针序列probe-c:ccccttaacgaagacag,序列5’段使用fam标记,3’端用mgb标记;特异探针序列probe-t:ccccttaatgaagacag,序列5’...
The primers used for the real-time RT–PCR were purchased from Takara. For laser microdissection-based RT–PCR, epithelial cells and stromal cells were separately collected from frozen sections of Gan mouse tumors (n ¼ 4) using laser microdissection LMD7000 (Leica Microsystems, Wetzler, ...
根据NCBI数据库中小鼠TNF-α和GAPDH的基因序列,应用Primer Premier 5.0软件设计正、反义引物和探针,引物和探针均由上海生工公司合成和修饰,见表1。 1.5 实时荧光PCR 总反应体系为25μL,包括cDNA模板2μL,50 mmo1/L MgC12溶液1.5μL,IMMOLASE DNA热启动酶0.2μL,100μmo1/L TNF-α及GAPDH正、反义引物和探...
5’-TGGTATGAAATGGCAAATCG-3’,特异性扩增片段为329bp);IL-1B引物(Forward Primer:5’-CTCATTGTGGCTGTGGAGAA-3’,Reverse Primer:5’-GGGATTTTGTCGTTGCTTGT-3’,特异性扩增片段为323bp);B-actin引物(Forward Primer:5’-GCCGTCTTCCCCTCCATCGTG-3’,Revere Primer:5’-TAC GACCAGAGGCATACAGGGACAAC-3’...
Gastric atrophy was evaluated with serum pepsinogens (PGI < 70ng/dl and PGI/II < 3), and TNF T-1031C and C-857T were genotyped by polymerase chain reaction with confronting two-pair primers (PCR-CTPP). Results The frequency of TNF-A T-1031C was 68.4% TT , 28.4% TC , and 3.3%...
The exon distribution and primer position of koala TNFα (a), IL10 (b) and GAPDH (c) genes when in alignment with other marsupial and human sequences of the corresponding genes.Marina, Mathew
primers: primer 1 (forward), 5′-GGAATTCATGAGGCAGTCTCTCCTATTCC-3′, and primer 2 (reverse), 5′-TTCCCACAAACTTTATTGCTTCTTTTCACTCGAT-3′; primer 3 (forward), 5′-GCTTGTTCATCGAGTGAAAAGAAGCAATAAAGTTTG-3′ and primer 4 (reverse), 5′-CGGGATCCCCGCACTCTGTTTCTTTGCTGTCA-3′.▵M-TACE ...
To determine whether higher TNF-α activity could be due to increased intracellular Ca2+, we performed polymerase chain reaction (PCR) analysis using specific primers for Inositol 1,4,5-trisphosphate receptor 1 (IP3R1) and IP3R3, predominantly expressed intracellular Ca release channels in ...
Primer sequences for internal controls were as follows: b-2-microglobulin—forward, CTGACCGGCCTGTATGCTAT, reverse, TATGTTCGGCTTCCCATTCT; glyceraldehyde-3- phosphate dehydrogenase—forward, GGCATTGCTCTCAAT GACAA, reverse, CCCTGTTGCTGTAGCCGTAT; and hypo- xanthine phosphoribosyl-transferase I—forward,...
Each mRNA quantification was normalized relative to the housekeeping gene hypoxanthine phosphoribosyl- transferase 1 (HPRT) using the primers; mHPRT forward, 50-GTCCCAGCGTCGT GATTAGC-30 and reverse, 50-TGGCCTCCCATCTCCTTCA-30. Expression vectors. The plasmids pcDNA3.1-Pellino3s-/3l-myc, Pellino...