Human TNFSF4 qPCR Primer Pair,即人TNFSF4 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是...
Human C1QTNF4 qPCR Primer Pair,即人C1QTNF4 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)...
TL1A/TNFSF15 qPCR Primers (1) Verified forward and reverse primers for analyzing the quantitative expression of gene. The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480. TL1A/TNFSF15 General Information ...
Quantitative PCR with reverse transcription (RT–qPCR) analysis of the EBV-B cells of P1 showed normal TNF mRNA expression (Extended Data Fig. 3c,d). Western blot analysis, using antibodies directed against the N terminus of TNF, of whole-cell lysates and supernatants from the EBV-B and ...
RT-qPCR and Western blot assay The specific procedure of RT-qPCR and western blot were conducted as previous descriptions [15]. The primer sequences and antibodies were listed in Table S4 and S1 respectively. Co-IP and ubiquitination assay The specific procedure of Co-IP was conducted as previ...
(RT–qPCR). Data are mean ± s.d. from three technical replicates from two mice.Pvalues by one-sided Welch’st-test.i,j, Immunofluorescence of P4 back skin sections depicting two different clones of TNF-expressing keratinocytes. Dashed lines indicate basal membrane.k–m, Immunofluorescence...
To investigate the potential function of macrophages in LIGHT-induced cardiac remodelling, we conducted qPCR analysis of macrophage migration- and polarisation-related genes, including MCP1, TNF-α, IL-1β, IL-10, TGF-β1, ARG1, and CD163, and found that the mRNA expression of MCP1, IL-...
A–B AKT and p-AKT protein detection was performed by western blot in MH7A cells with transfection of shNC/shRAC2 A or sh-NC/sh-METTL3/sh-METTL3+oeRAC2 B. *P < 0.05, ***P < 0.001 Supplementary Material 2: Supplementary Table 1 Primer sequences used for RT-qPCR...
Sequence of the qPCR primer pairs (all listed in the 5ʹ–3ʹ direction) are as follows: β-actin: F—CATCACCATCTTCCAGGAG; R—AGGCTGTTGTCATACTTCTC; CTGF: F—CAGCATGGACGTTCGTCTG; R—AACCACGGTTTGGTCCTTGG; CYR61: F—CTCGCCTTAGTCGTCACCC; R—CGCCGAAGTTGCATTCCAG; HK2: F—GAGCC...
Mouse Tnf qPCR Primer Pair,即小鼠Tnf qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一种...