根据qPCR和Western blotting的结果,经miR-18a抑制剂处理后,T98G细胞和GSC4D中RORA的表达明显上调(图6(D)和(E)),而在U87细胞和GSC2C中经miR-18a模拟处理后,RORA的表达明显上调(图6(D)和(E))。我们进一步进行了荧光素酶转运蛋白...
Results : ARPE-19 cells treated with 0.5% CSE for 4h showed significant increase in expression of the inflammation marker TNF-伪 and early EMT marker, Snail, expression by qPCR analysis (9-fold; p<0.01 and 4-fold; p<0.01, respectively). By 24h, both TNF-伪 and downstream inflammatory ...
因此,我们检测了RORA过表达或下调后TNF-α的表达变化。所有的qPCR、westernblotting和ELISA分析都表明,在RORA过表达后,TNF-a的表达和分泌被下调,而在RORA被敲除后,则得到相反的结果(图3(B)-(E))。 此外,我们还检测了我们的临床...
(e) RT–PCR and qPCR quantification of TNFR1 and TNFR2 expression in U937 monocytes, hLECs and HUVECs. *Po0.05; **Po0.01; ***Po0.001; NS, not significant. All error bars represent s.e.m. All P values were analysed according to Student's t-test. TNF-a stimulates lymphangiogenesis ...
RT-qPCR结果显示,Miz1缺失导致炎性因子和炎性小体成分mRNA表达增加。此外,在Miz1缺失组以及F/F和ΔHep原代肝细胞中,IL-1β、TNFα及其上游炎性体NLRP3的mRNA表达显著升高(图6A)。然后,为了确认Miz1是否通过增加线粒体自噬来调节NLRP3/IL-1β和NLRP3/TNF-α信号轴,作者用抑制线粒体自噬的药物阻断了线粒体...
Glioma stem cells (GSCs) are a special kind of cells in GBM showing tumor initiation, self-renewal, and multi-lineage differentiation abilities. Finding novel circRNAs related to GSCs is of great significance for the study of glioma. qPCR, western blotti
(2)成模后分别应用免疫组化、QPCR、western blotting检测随病程延长,TNF-a、VEGF、bFGF、IL-1β、IL-6的动态变化。(3)应用伊文思蓝渗透实验检测视网膜血管通透性的变化。2、细胞实验:(1)体外培养大鼠视网膜小胶质细胞,视网膜色素上皮细胞,视网膜血管内皮细胞,应用免疫荧光染色鉴定后作为实验对象。(2)将视网膜小胶质...
目的 研究茄病镰刀菌感染树鼩角膜过程中甘露糖受体和炎症因子的表达变化,探讨真菌感染性角膜炎发生机制.方法 通过接触镜辅助法用茄病镰刀菌感染树鼩角膜,前段照相,病理切片及活体共聚焦显微镜观察结果对动物模型进行评价.qPCR检测树鼩角膜炎病发过程中的甘露糖受体,dectin-1,IL-1β,IL-6,TNF-α的表达;体外培养树鼩原...
Porcine parvovirus; PRRSV: Porcine reproductive and respiratory syndrome virus; qPCR: Quantitative polymerase chain reaction; SC: Secreting cells; TCM: Central memory T cells; TEM: Effector memory T cells; TNF-α: Tumor necrosis factor alpha; VA: Vaccination group; VI: Vaccinated and infected group...
表現量上升;另外以qPCR確認MMP2、MMP9、E-cadherin的mRNA表現量皆與蛋白質表現量趨勢一致 ,而TNF-脉也能誘發EMT相關之轉錄因子ZEB-1之mRNA表現量增加,並且p65對此現象有 些微加乘作用;由以上結果得知,NF-κB可能部份參與在TNF-α誘發786-O產生EMT現象的路徑 ...