Notably, as shown in the heatmap, TNIP3 overexpression suppressed the biological processes related to fatty acid biosynthesis, elongation, transportation, and inflammatory response but increased fatty acid degradation (Figures 2D and 2E), which was further confirmed by quantitative PCR (qPCR) and ...
HyCyte™分子诊断标准品,基于肿瘤热门靶点,设置多种突变类型,适用于qPCR、一二三代测序等多种平台的性能评估。样本来源于人类细胞系,最大程度接近患者样本。覆盖多种癌症有关变异位点,包含肺癌、结直肠癌、乳腺癌、血液癌等。 | 产品特点 具有临床标本和肿瘤细胞的特性,无伦理风险; 有明确的突变频率,多重QC验...
The TNIP2 mRNA and protein levels were measured using reverse transcription quantitative polymerase chain reaction (RT‐qPCR) and western blot analysis, respectively. The caspase3 activity was calculated using a Caspase3 activity kit. Interleukin (IL)1β, IL‐6, and tumor necrosis factor‐alpha ...
10 IL-17a 8 6 4 2 5 IL-12a 4 3 2 1 IL-18 4 ** 3 2 1 0 Healthy CD (Active) 0 Control CD (Active) 0 Control CD (Active) Figure 9 Expression of tumor necrosis factor (TNF)-like cytokine 1A (TL1A) and CD161 is confirmed in quantitative polymerase chain reaction (qPCR) Crohn...
. We performed qPCR with 5 μL of the sample to determine the number of additional cycles required, while the rest remained on ice. The 5 μl of sample was added to a qPCR mix (5 μL of PCR, 5 μl of NEBNext, 0.5 μL F+R custom nextera primers, 0.09 μL ...
The qPCR was performed in triplicate; and the result is normalized to diluent control TNFa-induced apoptosis in PDA cells, we used lentiviral shRNA to generate HupT3 cells with stable knockdown of each gene and determined their response to TRAIL and TNFa (Figures 7a–c). Suppression of XIAP ...
TNF-α是由脂肪细胞、活化的单核细胞、巨噬细胞、B 细胞、T 细胞和成纤维细胞等多种细胞分泌的多效性促炎细胞因子。它属于 TNF 配体家族,通过两个受体 TNFR1 和 TNFR2 发出信号。TNF-α对多种肿瘤细胞具有细胞毒性,是介导细菌感染免疫应答的重要因子。TNF-α在感染性休克、自身免疫性疾病、类风湿性关节炎、炎...
Among the genes identified in this analysis, we selected NRN1 and TNFAIP3 to be evaluated for gene expression by RT-qPCR and DNA methylation by bisulfite DNA next-generation sequencing in 43 and 52 pairs of GC and adjacent non-neoplastic tissue samples, respectively. We identified 83 candidate...
First, qPCR demonstrated the same quantity of A20 DNA from the 3 types of plasmids, either directly from the plasmid or from the retrotranscription of A20 mRNA (supplemental Figure 4). Second, immunocytochemistry staining with an anti-FLAG-tagged antibody was able to detect the 3 types of A20...
Total RNA was extracted from CRC cells usingTRIzolreagent (Invitrogen, CA, USA). The expression levels of miRNAs were detected using the Hairpin-it™ miRNAs qPCR Kit (Genepharma, Shanghai, China). The expression of RNU6B served as an endogenous control. The expression of mRNA was measured ...