Regarding TNF signaling, TNF receptor TNFRSF1A as well as the TNF target gene TNFAIP3 were expressed by epithelial cells (Supplementary Fig. 7a), preferentially within differentiated colonocyte populations, including inflammatory colonocytes, in addition to secretory progenitors (Supplementary Fig. 7a)...
Results are expressed as mean ± standard error of mean (SEM). Following assessment for normality and equality of variances, statistical inferences on data were performed using one-way analysis of variance (ANOVA) followed by pairwise comparisons of treatment means using Dunnett’s post-hoc te...
qPCR primer probe TIRAP Reverse isoform B Applied biosystem 5′-AAGTAGATCAGATACTGTAGCTGAATCC Contact for Reagent and Resource Sharing Further information and requests for reagents may be directed to the Lead Contact, Jean-Laurent Casanova (jean-laurent.casanova@rockefeller.edu). Experimental Model and...
that functions as an adaptor protein connecting NLRP3 and caspase-1. NLRC4 (IPAF) gene was identified by Poyet et al.26when searching for proteins that can activate pro-caspase-1. They cloned IPAF cDNA using a pair of PCR primers based on sequence similarity to the CARD of pro-caspase-...
Quantitative real-time RT-PCR For miRNA RT-qPCR, cDNA was synthesised using TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) with miRNA-specific stem-loop primers. Mature miRNAs were detected on 7500 Real Time PCR System (Applied Biosystems) using TaqMan Universal Master Mix and ...
Approximately 1,100 proteins were >2-fold altered in expression in activated T cells (Figure 1C), including many proteins with established immune functions (e.g., IL2RA and TNFAIP3) (Figure 1D). Based on these results, we proceeded to use expanded T cells as the main control group in ...
that functions as an adaptor protein connecting NLRP3 and caspase-1. NLRC4 (IPAF) gene was identified by Poyet et al.26when searching for proteins that can activate pro-caspase-1. They cloned IPAF cDNA using a pair of PCR primers based on sequence similarity to the CARD of pro-caspase-...
TIMs with the Hallmark of TNFA signaling via NFkB and Inflammatory Response, and support our hypothesis that PGE2-EP4 signaling negatively and tonically controls the basal reactivity of the infiltrating myeloid cells of the M1-like state to various inflammatory stimuli in TME, and that inhibition of...
qPCR was conducted in triplicate in a total of 20 μL using PowerUp 2x SYBR Green master mix (Applied Biosystems), with a 60°C extension temperature, using the Quant Studio 3 instrument (Applied Biosystems). Data was analyzed by standard ΔΔCt method. Pan HLA qPCR primers were described...
Primer sequences are listed in Table S5. All qPCR experiments were performed using The StepOnePlus™ Real-Time PCR System (Applied Biosystems). Gene expression levels were normalized to GAPDH using the ΔCt method. Data were analyzed using one/two-tailed Student’s t test in Prism 8 (...