QH31753S Human PROCR qPCR Primer Pair 200次 22.00元 Human PROCR qPCR Primer Pair,即人PROCR qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real...
Primers for qPCR were obtained from Thermo Fisher Scientific. The relative expression level for each gene was normalized to that of GAPDH gene and subsequent fold changes were determined according to published methodology [51]. Western blot Cell lysates were prepared in standard radioimmunoprecipitation...
The transgene copy number in the transgenic mice was determined by qPCR as described previously16with primers designed for a single copy control gene (lymphotoxin B gene: Ltb) and the pBC1-INS transgene. The primer sequences for Ltb have been described previously16and the primer sequences for...
October 27, 2024 For double recognition of ALP and tartrate-resistant acidity phosphatase, the areas immunodetected for ALP were incubated in an assortment of 8?mg of naphthol AS-BI phosphate (Sigma-Aldrich), 70?mg of crimson violet LB sodium (Sigma-Aldrich), and 50?mM L (?+) tartari...
(GEM) incubation. P5 and P7 primers, Read 2, and the sample index were added during library construction via end repair, A-tailing, adaptor ligation, and amplification. The final libraries contained the P5 and P7 primers were used in Illumina® bridge amplification, and sequenced on the ...
Then a one-step real time quantitative polymerase chain reaction (RT-qPCR) kit (Land medical, China) with primers targeting the 5′UTR (263 bp) of HRVs was used to detect HRV RNA. The remaining viruses and mycoplasma were detected using an immunofluorescence assay kit (Diagnostic ...
Primers were designed to generate 100 - 150 bp amplicons and are listed in Table 3. qPCR was performed using Sybr Green mastermix (Invitrogen). Transcriptional differences were calculated using the ΔΔCt method [63]. Table 3 Oligonucleotides used in the study Full size table A. baumannii ...
Mutagenic primers to introduce ATG mutations, see Table S5 BioTeZ (Berlin, Germany) N/A Primers for circRNA validation RNase R qPCRs, see Table S7 Metabion (Munich, Germany) N/A Software and Algorithms BWA-MEM Li and Durbin, 2010 https://github.com/lh3/bwa dbSNP (v138) Sherry et al...
Real time (RT)-qPCR RNA isolation from lung punch biopsies was performed with the QIAGEN miRNeasy Micro Kit. cDNA and RT-qPCR were generated using the Qiagen SYBR green RT-PCR kit, as per the manufacturer’s instructions. The primer sequences used to determine mice collagen type I alpha 1...
(C) qPCR analysis of whole kidney lysates for the pro-angiogenic factor VEGF and for the fibrotic factors TGF-β and α-SMA. Data are mean ± SD of three independent experiments and are normalized to GAPDH mRNA and to 1 for normal and expressed as relative quantification (RQ). (D)...