Human PROCR qPCR Primer Pair 一键复制产品信息 产品编号:QH31753S 产品包装:200次 选择包装 200次 数 量 价格:¥22.00
For qPCR, cell harvest and mRNA extraction was performed according to manufacturer protocol (QIAGEN, Cat. No. 74,104). Briefly, 350 µl of RLT Plus buffer with 2 M final concentration of DTT (freshly added) was added and vortexed to make the cell lysate. Cell lysate was transferred to ...
(Invitrogen, Carlsbad, CA) was used for reverse transcription. qPCR was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Human heart RNA was purchased and used as a positive control. The sequences of the primers used in this study are presented ...
Populations were calculated using 16S qPCR rRNA analysis of the V1-V3 region of each of the groups and represented using the 2−ΔΔCT method relative to a reference sample and the total amount of bacteria as determined by the 27F/519R primer set. Across combination, the relative gene ...
Real-time qPCR was carried out in an Eppendorf MasterCycler RealPlex4 (Thermo Fisher Scientific) using the Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, no. 4367659), according to the manufacturer's instructions. The data were normalized to the endogenous GAPDH. Primer sequence was ...
In brief, specific reverse transcription (RT) reactions were performed with each microRNA primer and RNU44 as endogenous control using 10 ng of total RNA as input material. Following RT, qPCRs were run in 4 replicates using the Taqman Universal Mastermix (Life Technologies) on a Rotor-Gene ...
Briefly, kDNA amplification by qPCR was achieved usingt he kDNA minicircle-specific primers JW11 (CCTATTTTACACCAACCCCCAGT) and JW12 (GGGTAGGGGCGTTCTGCGAAA) (Nicolas et al., 2002). The qPCR reaction was performed using the Absolute Blue qPCR SYBR Green mix kit (Thermo scientific, Surrey,...
the first-strand cDNA was synthesised from 2 μg of total RNA with an optimised RevertAid™ First Strand cDNA Synthesis Kit (Fermentas, Germany), and the PCR was performed using Maxima™ SYBR Green/Fluorescein qPCR Master Mix (Fermentas, Germany) and forward and reverse primers for the res...
Therefore, we measured expression levels of these two genes in the two new mutants studied here using qPCR on RNA from the organ of Corti at P4, and both genes were found to be significantly downregulated in Mir96+13G>A and Mir96+14C>A homozygotes (Fig. 6)....
Quantitative PCR (qPCR) According to the manufacturer’s protocol, total RNA was isolated from iMG and retinal explants using Trizol reagent (Cat.# 15596018CN, Thermo Fisher Scientific). Subsequently, 1 µg of RNA was reverse transcribed to cDNA using HiScript III RT SuperMix for qPCR (+ ...