此外,奥拉帕利联合T-DXd治疗还抑制了MTOR基因的表达。后续筛选的其他TNBC PDX模型显示,WHIM3和WHIM4这两个相对抗T-DXd单药治疗的PDX模型在与奥拉帕利联合使用时也表现出耐药性。未来还需要进一步研究包括对这些PDX模型的基础及治疗后样本...
“在靶向治疗中,PARP 抑制剂的疗效最为显着,”Meric-Bernstam 指出。在用 talazoparib 治疗的 PDX 中,有 5 种病情消退,1 种疾病稳定至少 28 天。这五个 PDX 中有四个没有种系 BRCA1/2 突变,但在其他 DNA 修复途径中发生了改变,包括 ATM 缺失和 BRCA2 中的其他改变。 该团队正在开发 TNBC 的新 PDX...
The PDX collection includes models encompassing a broad heterogeneity of chemotherapy responses, histologic features, and molecular TNBC subtypes. In addition, the majority of models develop spontaneous lung metastases. Whole exome sequencing demonstrated conservation of mutations between patient tumors and ...
作者制作了一批TNBCs (15/18)的PDX模型,然后用这些模型来测试其对 EGFR inhibitorgefitinib敏感情况。前人报道该药物在TNBC病人里面有效率是38.7%,与他们的实验想符合(6/18), 但是其中有一个人的反映比较特殊,就是 GCRC1735,一个70岁的老奶奶,该药物治疗效果出奇的好。实验表明是正中靶点,降低了pEGFR,同时下调...
37 High levels of PRMT5 are associated with poor prognosis in TNBC23,34,35 and PRMT5 inhibition impairs tumor growth in a TNBC PDX model23 and xenograft models derived from TNBC cell lines.35,38 TNBC patients may therefore benefit from a treatment targeting PRMT5. As expec...
PDX and PDO experiments involved human breast cancer patients. The studies were approved by the Committee on the Use of Humans as Experimental Subjects of University of Kentucky, IRB protocol # 15–0767-P6H. All clinical samples were collected with written informed consent from patients in the Ma...
4.2. FABP4 knockdown inhibits BCSC activity and TNBC progression in patient-derived xenograft (PDX) model For enhanced clinical relevance, we established a patient-derived xenograft (PDX) model to comprehensively evaluate the expression and functional role of FABP4 in TNBC (Fig. 3A). By employing...
在患者来源的PDX模型中,联合PARP抑制剂奥拉帕利后,进一步增强了T-DXd抑制肿瘤生长和提高生存率的效果。此外,T-DXd与奥拉帕利的联合使用在促进DNA损伤及诱导细胞凋亡方面表现出更强的活性。进一步分析表明,联合治疗显著降低了mTOR、ATF4及与TNBC侵袭性相关的基因表达,这为之前观察到的T-DXd与奥拉帕利联合使用在抑制细胞...
when tested across a panel of 4 TNBC breast cancer cell lines. Considering LP-184’s in vitro anti-tumor activity for TNBCs, LP-184 was additionally tested in 10 patient derived xenograft (PDX) mouse models of TNBCs,...