PART 2: Previously we detailed patterns of tumor regression into a residual tumor state, followed by uncontrolled regrowth in the absence of treatment in multiple PDX models of TNBC. Although using PDX models necessitates immune-compromised mice, several types of stromal populations are present and ...
37 High levels of PRMT5 are associated with poor prognosis in TNBC23,34,35 and PRMT5 inhibition impairs tumor growth in a TNBC PDX model23 and xenograft models derived from TNBC cell lines.35,38 TNBC patients may therefore benefit from a treatment targeting PRMT5. As expec...
Human breast cancer PDX experiments Human breast cancer tissues were minced with scalpels until finely chopped, and then were mixed with Matrigel. The mixture of minced human breast cancer tissue with Matrigel was injected subcutaneously to the NOD-SCID IL2Rgammanull female mice (Jackson Laboratory)...
the pCR rate did not increase to 100% [17]. In a grade 3 TNBC patient-derived tumour xenograft (PDX) model in nude mice, mice engrafted with TNBC cells with high EGFR levels exhibited differential responses to EGFR-targeted drugs [19]. These findings suggested that...
In a grade 3 TNBC patient-derived tumour xenograft (PDX) model in nude mice, mice engrafted with TNBC cells with high EGFR levels exhibited differential responses to EGFR-targeted drugs [19]. These findings suggested that there might be an unknown mechanism leading to the activation of the ...
Incidentally, most such studies derived from patients and PDX and were focused on genetic or transcriptomic vicissitudes in TNBC, while proteomic and phospho-proteomic reprogramming in response to NACT and during the evolution of proliferating RD is not studied well in TNBC subtypes. Recently, we ...
Here we report studies of paired TNBC patient-derived xenografts (PDX) established before and after chemo-resistance. Despite significant genetic similarities, the chemo-resistant PDX model harbored a rare constitutively-active KRASQ61R mutation which was not present in the chemo-naive PDX. Further ...
We tested several PDX models for response to our compound. Treatment with KB-0742 resulted in TGIs of over 50% in several lymphoma models, including a TGI of 56% in 1 model of double-hit diffuse large B-cell lymphoma.These data support the continual development of KB-0742, which is ...
Here we utilize our novel decellularized patient-derived xenograft (PDX) TNBC model, TU-BcX-2K1 ("2K1") tumor scaffold, to test this hypothesis. We seeded MDA-MB-231-LKB1 overexpressed and MDA-MB-231-vector cells onto decellularized 2K1 PDX tumor scaffold or decellularized adipose tissue ("...
Therefore, for the first time, this study employed two different TNBC PDX tumors to examine the effects of the mRNA-NPs. mRNA-NPs are developed using EGFP-mRNA as a model and studied in TNBC cell lines, ex vivo TNBC PDX organotypic slice cultures, and in vivo TNBC PDX tumors. Our ...