Antibody Staining Double or Nuclear Staining Detection Deparaffinization and Rehydration Tip: Before moving to alcohol grades step, make sure to completely deparaffinize the sections. If the sections still have traces of wax, an additional immersion of 5 minutes in Xylene may be employed. Deparaffi...
This protocol provides instructions for use and troubleshooting guidelines for the Ce3D Tissue Clearing Reagents.
Crisp staining results require optimizing primary antibody dilution. Optimization of the labeling step with Alexa Fluor tyramides or Aluora reagents is crucial for getting high resolution images which enhances signal to background. If multiplexing fluorophores, please optimize the heat...
If R&D Systems' primary antibodies are employed, please refer to the product data sheets to obtain the recommended working dilutions. In the staining protocol, signal visualization is achieved using R&D Systems' fluorescent secondary antibodies and reagents. For all other reagents, pleas...
Your Data: LCM Staining Effects on Real-Time RT-PCR Typically, in order to isolate high quality RNA, the tissue has to be processed immediately after harvest. Ambion's patented RNAlater™ Tissue Collection: RNA Stabilization Solution ...
We describe two ways of handling samples: active perfusion with a pump, if the blood vessels can be connected with external tubes, and passive incubation on a shaker. We also outline the differences in the procedure that are dependent on the organ components, organ size, targeted labeling and...
Fat tissue was fixed in 10% neutral formalin, embedded in paraffin, and cut into 5-µm sections, which were either processed for hematoxylin and eosin (H&E) staining or immunostaining with a UCP1 antibody (1:500, Abcam, ab10983) as we previously described (Supplementary Table 1)16,37....
We assayed Ki67-staining to determine whether efferocytosing (AC+) macrophages were more proliferative than non-efferocytosing (AC−) macrophages in dex-mice versus phosphate buffer solution (PBS)-treated control mice. As expected, efferocytosing macrophages were uncommon in the thymi of PBS-...
Immunohistochemical stainings were performed with an automated immunostaining instrument (BenchMark ULTRA IHC/ISH Staining module, Ventana Medical Systems, Tucson, AR). The OptiView DAB IHC Detection Kit (OptiView, Ventana Medical Systems) was used based on the manufacturer's protocol. The procedure in...
Indeed, the NHS esters which lend adhesive functionality to the PU-PAA-NHS material can be compromised if exposed to blood, limiting the efficacy of the 3D printed patches in extremely bloody scenarios. To address this limitation, we leverage 3D printing to design a liquid-infused patch that ...