Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations. Cytometry A. 2005; 67A :4–17.Chow, S., Hedley, D., Grom, P., Magari, R., Jacobberger, J.W. and Shankey, T.V. (2005) ...
Starting with human whole blood, the CD3+ cell content of the isolated fraction typically ranges from 92.4 - 99.8% (as assessed by staining the start and isolated fractions with anti-CD3 or anti-CD2 antibodies, respectively). In the above example, the purities of the start and the final iso...
This Human Regulatory T Cell Whole Blood Staining Kit contains the buffers and monoclonal antibody necessary to successfully stainandRead more Have Questions? Quantity: 1 kit Catalog number88-8996-40 Price (USD)/ Each - 联系我们 Quantity:
上海玉博生物科技有限公司 主要致力于“Foxp3 Whole Blood Staining Kit (with PE PCH101) 1 eBioscience-88-8996-40”的生产销售。多年的“Foxp3 Whole Blood Staining Kit (with PE PCH101) 1 eBioscience-88-8996-40”生产与销售的经验,与各行业新老用户建立了稳定的合
This protocol was optimized for direct staining of human peripheral blood samples which is time-saving and mini- mizes variations in sample preparation. The procedures for previous studies on immunophenotyping assays were includ- ing isolation of PBMCs, freezing and thawing cells. How- ever, ...
Brief fixation of whole blood in 4% formaldehyde followed by treatment with Triton X-100 results in erythrocyte lysis and leukocyte light scatter and immunophenotypic features equivalent to those of other commercial lysis reagents. Intracellular pERK staining is significantly improved by treatment with me...
流式细胞术分析全血样本实验方案描述了在尽可能减少样本处理的情况下通过流式细胞术对全血样本进行免疫细胞分型。该方法不仅没有损害细胞结构和功能,而且能减少细胞损失[1,2]。可以直接在流式细胞仪上分析经细胞表面标志物抗体染色的全血样本。为方便...
Whole Blood Number Whole Blood Partial Thromboplastin Time Whole Blood Platelet Aggregation Whole Blood Platelet Concentrates Whole Blood Platelet Count After Operation Whole Blood Staining Device Whole Blood Sulfa Test Whole Blood Taurine Concentration Whole Blood Thrombin Time Whole Blood to Plasma ratio ...
1) whole blood staining 全血直接染色法1. Objective To compare peripheral blood mononuclear cells(PBMC) staining and fresh whole blood staining methods for detecting antigen-specific cytotoxic T lymphocytes(CTL) with MHC pentamers in chronic hepatitis B patients. 目的分别采用分离外周血单个核细胞(PBMC...
To evaluate the usefulness of autologous heparinized whole blood (WB) in preventing toxicity of Brilliant Blue (BB) dye and to compare the structural and functional outcome of use of WB with BB versus Conventional BB assisted internal limiting membrane (ILM) peeling in Macula Hole (MH) surgery....