Double or Nuclear Staining Detection Deparaffinization and Rehydration Tip: Before moving to alcohol grades step, make sure to completely deparaffinize the sections. If the sections still have traces of wax, an
Palmer, H. (2012) Standardized relative quantification of immunofluorescence tissue staining. Protoc. exch.Arques O, Chicote I, Tenbaum S, Puig I, G. Palmer H. Standardized Relative Quantification of Immunofluorescence Tissue Staining. Protocol Exchange 2012; doi:10.1038/protex.2012.008....
Tissue was not labeled with antibody Antibody is not compatible with 3D IHC Use antibodies validated for IHC and immunofluorescence staining. Over-fixation affecting access to antibody epitopes. Poor antibody labeling Antigen retrieval may be necessary to increase tissue permeability and antibody access to...
Next, we used histological staining and SEM to analyze the microstructure of cultured fish meats. Hematoxylin-eosin and immunofluorescence section staining showed that after 17 days culture, the muscle fibers exhibited a similar arrangement to the native muscle fibers (Fig.5dand Supplementary Fig.S5a)...
Cell proliferation and cell death can also be visualized through immunofluorescence microscopy, for example, with a TUNEL assay, or with a combined propidium iodide (PI) and syto 13 staining. PI stains all cell nuclei red, whereas syto 13 stains only intact nuclei green. The combination of ...
Immunofluorescence techniques, control of specificity and non-specific fluorescence Tissue homogenates The tissue is washed in physiological saline, cut into small pieces and washed several times in saline until free of blood. The tissue fragments are homogenized in an equal volume of physiological saline...
Immunofluorescence staining At the end of the chondrogenesis or growth culture, the final tissue constructs were washed with PBS and fixed with 95% v/v ice ethanol at 4 °C for 20 min. Afterwards, samples were permeabilized with 0.3% v/v Triton X-100 at room temperature for 20 min and ...
2.5. Immunofluorescence Staining After seeding and differentiation (3500 cells/cm2) onto glass slides, cells were grown until 85% confluence. They were then fixed with 4% paraformaldehyde (Sigma-Aldrich). After saturation and permeabilization (4% BSA (Sigma-Aldrich), 0.3% Triton X-100 (VWR Inte...
The protocol for GC/MS analysis of mono- and diterpenoids was based on previously published methods [6, 48]. Metabolites in 1 or 2 μl pentane (extracted from enzyme assays) or MTBE (extracted directly from tissue) were identified using a GC (Agilent 6890A series) coupled with a mass spe...
On one hand, kidney grafted with hepatic lobule-like cell cluster was also used to perform immunofluorescence staining for albumin. First, the tissue was placed in OCT-compound (Sakura Fineteck Inc.) and frozen immediately. A 7 μm section was prepared and fixed in 4% paraformaldehyde/PBS (...