Understand the different approaches to intracellular staining for flow cytometry in this "do-it-yourself" guide to fix and perm.Flow cytometry is a powerful technique used to identify groups of cells in a heterogeneous population using antibodies to measure relevant identifying markers. The detection ...
The Intacellular Flow Cytometry Staining Protocol describes the process for intracellular staining of various cell types (in vivo-stimulated tissues, in vitro-stimulated cultures, and whole blood) for flow cytometry using BioLegend's proprietary buffers
Procedures of Intracellular Staining Protocol Sample preparation 1. Harvest the tissues and cells, prepare a single-cell suspension and adjust the suspension to a concentration of 1 x 106 cells/mL in cell straining buffer. Learn more about sample preparation for flow cytometry. Note: Cell surface...
Here, we will discuss the various staining buffers that are used in flow cytometry. In addition, we have outlined detailed protocols for carrying out both surface and intracellular staining in human and rodent tissues such as whole blood and PBMCs. Specifically, for surface staining we outline ...
BestProtocols: Annexin V Staining Protocol for Flow Cytometry BestProtocols: BrdU Staining Protocol for Flow Cytometry BestProtocols: Cell Preparation for Flow Cytometry Protocols BestProtocols: UltraComp Compensation Beads Protocols for Flow Cytometry BestProtocols: Pharmacol...
Flow cytometry has become a basic of biological research and clinical diagnostics, and its application has been crucial to numerous advances in immunology and cell biology. However, several issues remain when considering intracellular stainings, especially in the context of a daily routine use and in...
Staining Intracellular Antigens for Flow Cytometry
Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer....
We describe here a method for optimizing the use of polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellular staining of human T lymphocytes. We will highlight and discuss how to procedurally optimize key steps in the experimental process before an intr...
After imaging the sample was removed from the flow cell and placed into 1x PBS prior to protein staining (Protein staining). After protein staining was complete, the sample was placed into the flow cell and filled with imaging buffer. The same region imaged during the smFISH experiment was ...