ObjectiveThe present study aims to explore the activation profile of THP-1 derived dendritic cells (TDDCs) stimulated by recombinant Mal f 1, as well as to explore the IgE-binding ability of rMal f 1 and its correlation with IgE-binding activity of complete allergens ofM. furfur. MethodsrMal...
TLR7 is mainly found in plasmacytoid dendritic cells (pDCs) and B cells, whereas TLR8 is highly expressed in monocytes, monocyte-derived DCs (mDCs), and macrophages [3]. TLR7 and TLR8 trafficking and signaling Upon viral infection, the chaperone protein UNC93B1 interacts with TLR7 and ...
TLR9 is mainly expressed in subsets of Dendritic Cells and in B cells of all mammals. In rodents, but not in humans, TLR9 is also expressed in monocytes and macrophages [3]. The structure of the receptor varies by 24% between human TLR9 (hTLR9) and mouse TLR9 (mTLR9) [3]. They rec...
Responsiveness of human monocyte-derived dendritic cells to thimerosal and mercury derivatives [J]. Toxicol Appl Pharmacol, 2010, 246(1/2): 66-73. [15] de Smedt A C A, van den Heuvel R L, van Tendeloo V F I, et al. Phenotypic alterations and IL-1beta production in CD34+ progenitor...
THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of macrophages or into dendritic cells. This chapter describes how to grow THP-1, resp. U937 cells, how to differentiate these into more specialised phenotypes such as various...
Zoledronic acid modulates maturation of human monocyte-derived dendritic cells. Exp Biol Med. 2011;236:1420- 1426. 37. Sansoni P, Passeri G, Fagnoni F, et al. Inhibition of antigen- presenting cell function by alendronate in vitro. J Bone Miner Res. 1995;10:1719-1725. 38. Recenti R,...
THP-1巨噬细胞形态图;b. 实时荧光定量PCR检测PMA对THP-1细胞CD68表达的影响;c. 流式细胞技术检测PMA对THP-1细胞CD68表达的影响;d. CD68相对荧光强度统计分析**p < 0.01) Fig. 1Effects of BPA on differentiation of THP-1 cells to macrophages. (a. Morphology of THP-1 macrophages; b. THP-1 cells...
THP-1 monocytes are described to differentiate into mature dendritic cells by transferring them to serum-free medium, and subsequently treating them with a mixture of IL-4, GM-CSF, TNF-α and ionomycin. These hematopoietic cell line-derived DCs are highly pure and monotypic, and display the mo...
Furthermore, an enhanced mesenchymal stem cell recruitment by dendritic cells derived EVs was reported36. In the context of reproductive immunology, it has been described that (1) placenta-derived EVs in co-culture with non-pregnant peripheral blood mononuclear cells (PBMCs) bound to monocytes and...
sensing viral-derived components and are mainly found in the endosome. Among these, TLR3recognizes double-stranded (ds)RNA, a hallmark of viral replication, and triggers antiviral immune responses [1]. TLR3 is expressed in myeloid dendritic cells, macrophages, as well as non-immune cells [2]...