THP-1 monocytic cellsPMA differentiationWhen THP-1 cells are differentiated into adherent macrophage-like cells, they respond to inflammatory stimuli by changing their phenotypes to an activation state and altering the expression of inflammation-related genes. Nitric oxide (NO) is a diatomic molecule ...
N A Re, I M Carr, etc. Standardized protocols for differentiation of THP-1 cells to macrophages...
cohol (PVA), with U937 and THP-1 cells. Meas- urement of differentiation into macrophages will be analyzed by means of biochemical and mor- phological assays. MATERIALS AND METHODS Cell culture - THP-1 cells, leukemic cell line de- ...
Optional: PMA-induced THP-1 Differentiation Following Phorbol 12-myristate 13-acetate (PMA) treatment, THP1‑Dual™ cells are more sensitive to IFN-inducers, such as transfected Poly(dA:dT), while the response to certain TLR ligands, such as Pam3CSK4, is diminished. Note: PMA treatment ...
CommentsThe cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Culture Method ...
adherence and altered cell function. Nitric oxide (NO), an intracellular messenger, is critical in regulating cell differentiation. Here we elucidated whether NO relates to PMA-induced monocyte-to-macrophage differentiation of THP-1 cells. The mutual regulation of calcium and NO was also investigated...
THP-1 cells have been shown to spontaneously phagocytose bacteria without the need for activation or differentiation20. To establish THP-1 cells as a screening model which ensures homogenous Cas9 expression and thereby a high dynamic range of effect sizes in CRISPR screens21, we engi- ...
THP-1 cells were cultured for 24 h in medium containing 50 ng/mL PMA (Sigma-Aldrich, USA), which was used to induce THP-1 monocyte differentiation into macrophages. Adherent cells were then infected with BCG at an MOI of 10. Construction and transfection of DUSP1 small interfering RNAs ...
The innate immune system senses intracellular double-stranded RNA (dsRNA), a replication intermediate for RNA viruses, through two RNA helicases: retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-association gene 5 (MDA5). Upon recognition of dsRNA, RIG-I and MDA5 are ...
14.7Differentiation of THP-1 and U937 Monocytes into Dendritic Cells THP-1 monocytes are described to differentiate into mature dendritic cells by transferring them to serum-free medium, and subsequently treating them with a mixture of IL-4, GM-CSF, TNF-α and ionomycin. These hematopoietic cell...