ligase (redirected fromligase chain reaction) Medical Acronyms Encyclopedia li·gase (lī′gās′, -gāz′) n. Any of a class of enzymes that catalyze the linkage of two large molecules. [Latinligāre,to bind; seeleig-inIndo-European roots+-ase.] ...
& Oda, J. A pseudo-Michaelis quaternary complex in the reverse reaction of a ligase: structure of Escherichia coli B glutathione synthetase complexed with ADP, glutathione, and sulfate at 2.0 A resolution. Biochemistry 35, 11967–11974 (1996). Article CAS PubMed Google Scholar Berg, H. ...
The reaction was reversible, and Phe-tubulin amount was a function of both Phe concentration in medium and incubation time. The ability of Phe-tubulin to undergo assembly into microtubules was demonstrated in differentiated CAD cells (Fig. 3), and cell viability was not altered by Phe ...
Real-time quantitative polymerase chain reaction (RT-qPCR) RT-qPCR assays were used to detect the amplification of specific genes. The total RNA from the basal ganglia of the mouse brain was reverse transcribed into cDNA using a first-strand synthesis kit (Vazyme, China) according to the manufa...
The reaction product was connected to the 50-RACE adaptor using T4 RNA ligase. With the ligation product as template, the first- strand cDNA was synthesized using random 9-mers and M-MLV reverse transcriptase (RNase H-). Both outer and inner 50-RACE PCR reactions were performed as follows:...
Derlin1 was key component protein in the ubiquitin ligase complexes (Zattas et al., 2013). Derlin1 was significantly higher in the group 30℃ for 6 h, which implied more requirement for protein ubiquitination and degradation. Taken together, the differential expression patterns of DEGs in ER ...
3). Oxidation without light emission is known to occur approximately 20% of the reaction, but it occurs more often when firefly luciferase without the C-terminal domain is used in the reaction [28], [29]. In FLCA, firefly luciferase is split into the N-terminal domain (NFLuc thereafter)...
(100 ng) and 1 μL of 3′SR adaptor for Illumina in a total volume of 7 μL. The mix was incubated in a pre-heated thermal cycler for 2 min at 70 °C and a transfer tube on ice. After, the following components were added: 10 μL of 2X 3′ Ligation Reaction ...
In the GA signalling pathway, the receptor GID1 and E3 ligase SCFSLY1/GID2 together promote the degradation of the DELLA repressor proteins in a GA-dependent manner to relieve their repression of GA action12,13,14. Although recent studies have shown that ABA can antagonize GA-promoted ...
Bulk RNA-seq results were validated via quantitative polymerase chain reaction (qPCR) (Supplementary Fig. 1c). Our results revealed that the gene profiles of microglia from the female brain gradually changed during the aging process (Fig. 1b), indicating a progressive aging. Principal component ...