Diagram The diagram below shows the concept behind the TA Cloning®method. Thermostable polymerases containing extensive 3´ to 5´ exonuclease activity, such as Platinum®Pfx, do not leave 3´ A-overhangs. PCR products generated with Taq polymerase...
a Schematic diagram of ZMYM family proteins. b In vitro interaction assay between CRBN and ZMYM2, ZMYM3 or ZMYM4. The interaction between bls-CRBN and FLAG-GST-ZMYM2, -ZMYM3 or -ZMYM4 in the presence of DMSO or 20 µM thalidomide (Th), lenalidomide (Le), pomalidomide (Po) or ...
B Venn diagram demonstrating the intersection of overexpressed NAP1L1 (mass spectrometry data) and BIRC2-associated proteins. C Co-IP assay revealed that NAP1L1 could bind to UBR4. D Co-IP assay revealed that BIRC2 could bind to UBR4. E, F Western blotting was performed to evaluate the ...
The following illustrated diagram depicts how a double stranded DNA helix is unwound by topoisomerase, unzipped by helicase, and synthesized by DNA polymerase. New strand synthesis occurs on both strands of DNA during replication. Notice how, when moving from one end to the other, each strand is...
(A) The schematic diagram represents the architectural alignment of all the three domains (TPR, Ubox and Charged) of CHIP. TPR (tetratricopeptide repeats) domain is present at the N-terminus of CHIP, while Ubox domain is at C-terminus. The charged domain is localized in between TPR and ...
(A) Schematic diagram of the structure (top) and predicted amino acid sequence (bottom) of mouse SLIM. PDZ and LIM domains are boxed and conserved cysteine and histidine residues in the LIM domain are underlined. (B) Northern blot analysis of SLIM expression in mouse tissues (left) and prim...
In both cases, successive addition of activated ubiquitin moieties to internal Lys48 on the previously conjugated ubiquitin moiety leads to the synthesis of a polyubiquitin chain that serves as the degradation signal for the 26S proteasome A UPSAutophagyReview ...
a Schematic diagram showing real-time ubiquitination reaction results in loss of FRET between the Ubc13~Ub conjugate and transfer of Cy3B-labelled ubiquitin on to the substrate that diffuses into solution. Excitation (Ex) and emission (Em) wavelengths are highlighted. b Images of Cy3B and Alexa...
FIG. 1 is a flow diagram depicting polymerase chain reaction (“PCR”)/ligase detection reaction (“LDR”) processes, according to the prior art and the present invention, for detection of germline mutations, such as point mutations. FIG. 2 is a flow diagram depicting PCR/LDR processes, acco...
50]. Thus, we measured the transcript levels of fourPRgenes,PR1a,PR1b,PR10andPBZ1, by reverse transcription polymerase chain reaction (RT-PCR) analysis. We found that the expression of all these genes