The ligase chain reaction (LCR) is a form of probe-based amplification that involves thermal cycling. Sample dsDNA is denatured by heat to expose a single-stranded target sequence ( amplicon ); the temperature is then lowered, and two specific probes (oligonucleotides, oligomers) anneal to the ...
Like thermostable DNA polymerase, thermostable ligase is tolerant to high temperatures (approximately 90–100 °C) (Barany, 1991) and can keep catalytic activity among 30–80 °C (Mathur et al., 1996), which makes it appropriate for thermocycling reaction. Ligase chain reaction (LCR) exploits ...
Such thermostable DNA ligases retain catalytic activity in nick-binding reactions during temperature cycling, including thermal (90–100°C) denaturation steps of double-stranded DNA. Thermostable DNA ligases are important as ligase detection reaction (LDR)/ligase chain reaction (LCR) enzymes. LDR/LCR...
- 连接扩增(连接酶链式反应、LCR) - Gibson Assembly 克隆 - 重复扩增检测(RED) - 同时突变多个位点 产品类型DNA连接酶试剂盒 推荐用于高耐热性DNA连接酶,适用于高温连接有优势的应用 组成成分Ampligase DNA Ligase Kit contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. ...
产品应用- Ligation amplification (Ligase Chain Reaction, LCR) - Repeat Expansion Detection (RED) - Simultaneous mutagenesis of multiple sites - Other ligation-based detection methods - 连接扩增(连接酶链反应,LCR) - 重复扩展检测(RED) - 多个位点同时诱变 ...
Oligonucleotide products are exponentially amplified by thermal cycling of the ligation reaction in the presence of a second set of adjacent oligonucleotides that are complementary to the first set and the target. A single-base mismatch at the junction prevents ligation and amplification, thus ...
This approach is referred to as ligase chain reaction (“LCR”). F. Barany, “Genetic Disease Detection and DNA Amplification Using Cloned Thermostable Ligase,” Proc. Nat'l Acad. Sci. USA, 88:189-93 (1991) and F. Barany, “The Ligase Chain Reaction (LCR) in a PCR World,” PCR ...
The availability of high fidelity thermostable ligases enables the ligation reaction to be performed in a thermocycling format. Ligase detection reaction (LDR) employs one pair of DNA probes. Continuous target denaturation, probe annealing and strand joining linearly amplify a target sequence. Ligase ...
Oligonucleotide products are exponentially amplified by thermal cycling of the ligation reaction in the presence of a second set of adjacent oligonucleotides that are complementary to the first set and the target. A single-base mismatch at the junction prevents ligation and amplification, thus ...