a上海贸易发展公司 正在翻译,请等待...[translate] aIf a ligation reaction has been carried out using a vector and target prepared with a single restriction enzyme 如果结扎术反应使用传染媒介和目标被执行了准备与唯一制约酵素[translate] a他们在谈论什么? What are they discussing?[translate]...
The three steps of a DNA ligation reaction (Figure 1): The DNA ligase is self-adenylated by reacting with ATP or NAD+, creating a phosphamide bond between a lysine residue within the enzyme and an AMP molecule of the cofactor, either ATP or NAD+. The adenyl group is transferred to 5...
aIn transformation of E. coli, a solution of a plasmid molecule, or a mixture of molecules formed in a ligation reaction, is combined with a suspension of competent cells for a period, to allow the DNA to be taken up. 在E.的变革。 杆菌,质粒分子的解答或者分子混合物在结扎术反应形成了,与...
a我就喜欢这样说话 I like speaking like this [translate] aThe linear vector will hence be unable to ligate into a circle, since no phosphates are available for the ligation reaction (Fig. 2). 因此线性传染媒介无法绑扎入圈子,因为磷酸盐为结扎术反应不是可利用的(。 2). [translate] aconnections...
Ligations- These digested fragments are then ligated to a compatible vector using an automated ligation reaction, facilitated by systems like robotic pipetting or liquid handling robots. Transformation- The ligated DNA is subsequently transformed into competent cells via automated electroporation systems, us...
Complexity is not important for subcloning of a single insert into a vector; therefore, you can accomplish this with a much shorter reaction time, 15 minutes or less, over a much broader range of temperatures, 16 degrees Celsius to 37 degrees Celsius.Read More...
This intermediate reacts with a second ketoacid to form acetolactate or acetohydroxybutyrate as products. Whereas the mechanism involved in the formation of HEThDP(-) from pyruvate is well understood, the role of the enzyme in controlling the carboligation reaction of HEThDP(-) has not been ...
Is it better for a man to get fixed or a woman? Vasectomies are cheaper, faster, andsafer than female sterilization, yet only 9% of men in the U.S. get them while 27% of women get tubal ligations. Comparing the risks and benefits of vasectomy versus tubal ligation needs to be consid...
aIn transformation of E. coli, a solution of a plasmid molecule, or a mixture of molecules formed in a ligation reaction, is combined with a suspension of competent cells for a period, to allow the DNA to be taken up. 在E.的变革。 杆菌,质粒分子的解答或者分子混合物在结扎术反应形成了,与...
aCentrifuge the ligation reactions briefly. Add 2μl of each ligation reaction to a sterile 1.5ml tube on ice. Prepare a control tube with 0.1ng of uncut plasmid. 简要地分离结扎术反应。 增加每结扎术反应2μl到一支不育的1.5ml管在冰。 准备控制管与未割减的质粒0.1ng。 [translate] aSet up ...