Isotope-labeled internal standards were monitored in each sample to ensure proper MS sensitivity for quality control. Pooled plasma samples were interspersed at intervals of 20 participant samples in the HILIC method and intervals of 10 participant samples in the amide chromatography method to enable...
Salt-overly-sensitive 1 (SOS1) is a unique electroneutral Na+/H+ antiporter at the plasma membrane of higher plants and plays a central role in resisting salt stress. SOS1 is kept in a resting state with basal activity and activated upon phosphorylation.
We therefore compared the Triton solubility of cholesterol in the different viral envelopes and the host cell membrane. BHK cells were labeled with [3H]cholesterol, and viruses were produced and purified. The radiolabeled membranes were extracted with Triton X-100 on ice and centrifuged, and ...
Analysis was completed with the appropriate horseradish peroxidase labeled secondary antibody (donkey anti-goat IgG or goat anti-rabbit IgG), and ECL detection system (GE Healthcare). The Image J program (v1.4) was used to quantitate proteins. For immunoprecipitation studies, plasma membrane ...
The product was then labeled with digoxigenin-dUTP. The membrane was hybridized with the probe at 42°C for 18 h. After stringent washing and immunological detection, the hybridization bands were visualized. Expression analysis of GhAPm via real-time PCR Real-time PCR was conducted using SYBR ...
Distortion of homeostatic signaling proteins by simulated microgravity in rat hypothalamus:A(16) O/(18) O-labeled comparative integrated proteomic approach. Proteomics, 2014; 14, 262-73. doi: 10.1002/pmic.201300337 [26] Iqbal J, Li W, Hasan M, et al. Differential expression of specific ...
The average of four labeled samples mixes was used as reference (REF), based on the weighted average of the intensity of report ions in each identified peptide. The final ratios of protein were normalized by the median average protein ratio for mixes of different labeled samples (7 d/REF, ...
Cell surface retention sequences (CRS) are polybasic domains implicated in membrane localization [19, 20]. They appear to be homologous to NLSs and may function as such when appropriate. There is evidence that adjacent sequences may be important in targeting a protein to the plasma membrane or...
Fig. 3: Highly enriched membrane-bound IgM molecules on the tips of MV of human B cells. a–c Representative 4x-ExM-Airyscan images of a B cell labeled with anti-CD45-AF488 (green; a), anti-IgM-CF633 (magenta; b), and the merged image (c). Images in a–c are representative of...
membrane LAP240stained the NE only in Triton X-100-permeabilised spermatids (see below), whereas the antibody to the outer nuclear membrane marker SYNE1 labeled the spermatid NE after selective digitonin permeabilization (see below). Of importance, we noticed that the SUN5-labeling pattern in ...