8B). Cell identification was performed with an image of the plasma membrane labeled with wheat germ agglutinin (WGA)/Alexa Fluor 555 conjugate (upper left, Fig. 8B). Note: the image also had the Golgi apparatus and F-actin network labeled38. The plasma membrane was traced using the polygon...
(pp. 1484–1499) report that ROPs are targeted to the plasma membrane not only through the activity of RhoGDIs but also directly by vesicular trafficking, involving COPII, ARF1, AP‐1, and sterols. RhoGDIs and the vesicular trafficking route synergistically mediate ROP targeting and thus cell...
Multiplexed human tonsil tissue labeled with a panel of 8 OPAL dyes and DAPI acquired on a STELLARIS system, cell segmentation performed with Aivia 14. Advanced data analysis accessible for all When working in a core imaging facility or as an academic investigator in cell biology or neurosciences...
产品类型细胞涂覆试剂盒 产品规格试剂盒 产品线Image-iT™ 数量足够用于十块384或96孔板 子细胞定位Plasma Membrane, Nucleus, Mitochondria, Cytoplasm, Cytoskeleton, Golgi, Endoplasmic Reticulum, Nucleolus 标签类型Fluorescent Dye Unit SizeEach 内容与储存 足够用于十块384或96孔板图表...
1 The JUMP-MOA plate map labeled by compound. The JUMP-MOA compound plate map: unlabeled wells are DMSO only; all other wells are labeled to show the distribution of compound replicates across the entire plate. A version of this figure grouped by MOA rather than compound is available as ...
Figure 2. Phenotype comparisons of untreated vs. pharmacological control exposure in U2OS cells.Cells were treated with compounds of interest at 1–100 μM final concentrations for 48 hours in 96-well imaging plates. After treatment with the compounds, cells were immediately labeled using the Image...
1-30C). The lamellae or disks in cones are not surrounded by a plasma membrane in the same manner as rods but are in free communication with the interphotoreceptor space. Cone disks have a greater lifespan than rods and are not produced in the same manner; in addition, they do not ...
Segment (or infer) the cellmask and nuclei from an image depending on the type of fluorescent labels used and the number of cells per field of view (FOV): Fluorescently labeled nuclei (no cell or plasma membrane) one or more cells per FOV No cell, plasma membrane, or nuclei labels ...
making it more challenging to maintain cell health for the duration the experiment. The image below was captured from live HeLa cells labeled with 4 different fluorophores to identify nuclei, actin, tubulin and plasma membrane. With STELLARIS, it was possible to collect all 4 channels in a singl...
Fluorescently labeled actin fibers were identified using a Unet-ResNet50 deep learning model and skeletonized. Cell masks consisting of concentric “rings” were generated through fixed width areas starting at the cell edge. These rings were used to calculate the tilt of fibers (that is the angle...