1-30C). The lamellae or disks in cones are not surrounded by a plasma membrane in the same manner as rods but are in free communication with the interphotoreceptor space. Cone disks have a greater lifespan than rods and are not produced in the same manner; in addition, they do not ...
Alone or in multiplex studies with the reactive Alexa Fluor® or pHrodo™ dyes mentioned above, CellLight® fluorescent proteins can be used to label early or late endosomes, the plasma membrane, cytoskeletal p...
various techniques for statistical platelet aggregate analysis. The FDM microscope’s image acquisition of all platelet events (e.g., single platelets, platelet-platelet aggregates, and platelet-leukocyte aggregates) was triggered by detecting fluorescence signals from anti-CD61-PE-labeled platelets (se...
Live HeLa cells have been labeled to identify nuclei (cyan, Hoechst), actin (yellow, SPY555), tublin (magenta, SPY650) and plasma membrane (gray, NIR750). LAS X Navigator – a GPS for your experiment Intuitive sample navigation tools are essential to assure optimal setup of complex ...
Segment (or infer) the cellmask and nuclei from an image depending on the type of fluorescent labels used and the number of cells per field of view (FOV): Fluorescently labeled nuclei (no cell or plasma membrane) one or more cells per FOV No cell, plasma membrane, or nuclei labels ...
(pp. 1484–1499) report that ROPs are targeted to the plasma membrane not only through the activity of RhoGDIs but also directly by vesicular trafficking, involving COPII, ARF1, AP‐1, and sterols. RhoGDIs and the vesicular trafficking route synergistically mediate ROP targeting and thus cell...
may include a number of different organelles or compartments, organized for a number of different functions, including the nucleus, mitochondria, chloroplasts, lysosomes, peroxisomes, vacuoles, Golgi apparatus, rough and smooth endoplasmic reticulum, centrioles, plasma membrane, nuclear envelope, endosomes...
Finally, fluorescent labeling can lead to immunogenicity and introduction of xenobiotic compounds, such that human cells including human induced pluripotent stem cells (hiPSCs)12 and chimeric antigen receptor T (CAR-T) cells13 cannot be fluorescently labeled before in vivo use as cell therapies14. ...
Fluorescently labeled actin fibers were identified using a Unet-ResNet50 deep learning model and skeletonized. Cell masks consisting of concentric “rings” were generated through fixed width areas starting at the cell edge. These rings were used to calculate the tilt of fibers (that is the angle...
Multiplexed human tonsil tissue labeled with a panel of 8 OPAL dyes and DAPI acquired on a STELLARIS system, cell segmentation performed with Aivia 14. Advanced data analysis accessible for all When working in a core imaging facility or as an academic investigator in cell biology or neurosciences...