8B). Cell identification was performed with an image of the plasma membrane labeled with wheat germ agglutinin (WGA)/Alexa Fluor 555 conjugate (upper left, Fig. 8B). Note: the image also had the Golgi apparatus and F-actin network labeled38. The plasma membrane was traced using the polygon...
Imaging fluorescently labeled caveolin at the plasma membrane with total internal reflection fluorescence (TIRF) microscopy reveals a spatially heterogeneous distribution with aggregates of various sizes. In this chapter, we present a set of image-processing tools to quantify the spatial organization and ...
Multiplexed human tonsil tissue labeled with a panel of 8 OPAL dyes and DAPI acquired on a STELLARIS system, cell segmentation performed with Aivia 14. Advanced data analysis accessible for all When working in a core imaging facility or as an academic investigator in cell biology or neurosciences...
1 The JUMP-MOA plate map labeled by compound. The JUMP-MOA compound plate map: unlabeled wells are DMSO only; all other wells are labeled to show the distribution of compound replicates across the entire plate. A version of this figure grouped by MOA rather than compound is available as ...
子细胞定位Cytoskeleton , Plasma Membrane, Golgi, Mitochondria, Cytoplasm, Nucleus, Endoplasmic Reticulum, Nucleolus 颜色Multicolor 适用于(应用)High-content Screening (HCS), Morphological Cell Profiling, Multiparameter Screening, Fluorescent Labeling, 3D Labeling 适用于(设备)Fluorescence Microscope , High Con...
Figure 2. Phenotype comparisons of untreated vs. pharmacological control exposure in U2OS cells.Cells were treated with compounds of interest at 1–100 μM final concentrations for 48 hours in 96-well imaging plates. After treatment with the compounds, cells were immediately labeled using the Image...
Segment (or infer) the cellmask and nuclei from an image depending on the type of fluorescent labels used and the number of cells per field of view (FOV): Fluorescently labeled nuclei (no cell or plasma membrane) one or more cells per FOV No cell, plasma membrane, or nuclei labels ...
Details of the Microfluidic Chip and Optics-Microfluidic Integration, Related to Figure 1 (A) Schematic of the microfluidic chip. (B) Concept of continuous sorting with the on-chip dual-membrane push-pull sorter. (C) Schematic of the optics-microfluidics integration unit. (D) Installation of ...
1-30C). The lamellae or disks in cones are not surrounded by a plasma membrane in the same manner as rods but are in free communication with the interphotoreceptor space. Cone disks have a greater lifespan than rods and are not produced in the same manner; in addition, they do not ...
3A-3B, showing the ring-like structure of the opening of the CCPs at the plasma membrane, while FIG. 3E is a magnified view of the 100 nm thick x-y cross-section of two nearby CCPs. The circular ring-like structure of the pit periphery was unambiguously resolved. The consecutive x-y ...