The plasma membrane structure is a lipid bilayer that separates the interior of the cell from the exterior of the cell. Here we describe cell membrane markers that outline the cell boundary for fluorescence imaging. Fluorescent labeled lectins (e.g., wheat germ agglutinin (WGA)) can identify ce...
Cells experience a variety of perturbations on the plasma membrane, ranging from physical attack to pathogen invasion1. The plasma membrane is also damaged by physiological activities, including muscle contraction1,2,3. There is a growing appreciation that failed plasma membrane damage (PMD) response ...
We examined the structure of the affinity-labeled insulin receptors in rat brain, rat liver, and human IM-9 lymphocytes using sodium dodecyl sulfate-polyac... S.,Anne,Hendricks,... - 《Journal of Neurochemistry》 被引量: 259发表: 1984年 Expression of insulin-like growth factor receptors I ...
Live-cell F-actin and plasma membrane labeling F-actin was labeled with 1 μM of the live-cell Spirochrome probe SiR-actin (Cytoskeleton) which is based on natural actin-binding jasplakinolide. The plasma membrane was labeled with CellMask Green using the manufacturer’s recommended 1x working...
Protein Half-Lives of Calmodulin and the Plasma Membrane Ca-ATPase in Rat Brain We report the half-lives for two proteins involved in the regulation of calcium in the brain: the Ca-ATPase and its regulatory protein, . -labeled leucine ... DA Ferrington,X Chen,AG Krainev,... - 《Biochemi...
The plasma membrane is a convenient marker of cell boundaries and as such, a number of probes have been used for staining of the membrane. Typically, dyes of a lipophilic nature are used; however, they internalize rapidly, offering a very narrow window for imaging. Fluorescently labeled lectins...
Rather, a significant amount of the fucose-labeled glycoprotein fractionates with an internal membrane system(s) of this cell. The fucose-containing glycoproteins in the different cell fractions were compared by electrophoresis in dodecyl sulfate-polyacrylamide gels; both the internal membrane system and...
Notably, aged sEVs injected into aged mice should have been labeled to assess the presence of donor aged sEVs across various tissues, mirroring the approach used for donor young sEVs. This additional step would have facilitated an investigation into whether aging affects the tropism of circulating...
The plasma membrane is a convenient marker of cell boundaries and as such, a number of probes have been used for staining of the membrane. Typically, dyes of a lipophilic nature are used; however, they internalize rapidly, offering a very narrow window for imaging. Fluorescently labeled lectins...
Mixed lineage kinase domain-like protein (MLKL) was identified to function downstream of receptor interacting protein 3 (RIP3) in tumor necrosis factor-α (TNF)-induced necrosis (also called necroptosis). However, how MLKL functions to mediate necroptosi