The covalent reaction of the fluorescent dye with cysteine residues of proteins allowed the visualization of the labeled cell compartment by microscopy, as well as in membrane preparations of cell homogenates from corn, squash and carrots. Density gradients from a resuspended membrane pellet (1000–15...
CellMask™ plasma membrane stains are designed to deliver uniform staining of the plasma membrane across a wide variety of mammalian cell types and are slow to internalize, particularly compared to traditional approaches such as DiI, DiO, and labeled wheat germ agglutinin.CellMask™ plasma ...
Grunberger D, Haimovitz R, Shinitzky M (1982) Resolution of plasma membrane lipid fluidity in intact cells labeled with diphenylhexatriene. Biochim Biophys Acta 688:764-774D. Grunberger, R. Haimovitz, M. Shinitzky, Resolution of plasma membrane lipid fluidity in intact cells labeled with ...
CellMask™ plasma membrane stains are designed to deliver uniform staining of the plasma membrane across a wide variety of mammalian cell types and are slow to internalize, particularly compared to traditional approaches such as DiI, DiO, and labeled wheat germ agglutinin.CellMask™ plasma ...
It is unclear whether the establishment of apical–basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determ...
CellMask ™ Orange,DeepRed,andGreenplasmamembranestainsdeliveruniform stainingoftheplasmamembraneacrossawidevarietyof mammaliancelltypesand areslowtointernalize,particularlycomparedto traditionalapproachessuchasDiI, DiO,andlabeledwheatgermagglutinin. TheCellMask ...
Moreover, the membrane protein interactome in CPCs showed a strong relationship with biological processes related to cell adhesion whereas the BM-MSCs interactome was more related to immune regulation processes. We conclude that the stronger capacity of CPCs over BM-MSCs to engraft in the infarcted...
Plasma Membrane Proteomics of Human Embryonic Stem Cells and Human Embryonal Carcinoma Cells Human embryonic stem cells (hESCs) are of immense interest in regenerative medicine as they can self-renew indefinitely and can give rise to any adult cell......
1990. Plasma-membrane transport of alanine is rate-limiting for its metabolism in rat-liver parenchymal cells. FEBS Lett. 119:271- 74Sips HJ, Groen AK, Tager JM (1980) Plasma-membrane transport of alanine is rate-limiting for its... HJ Sips,AK Groen,JM Tager - 《Febs Letters》 被引量...
Cholesterol synthesized from [H] acetate moved to the plasma membrane with a half-time of 1 h at 37C. They used equilibrium gradient centrifugation of homogenates of biosynthetically labeled, cholesterol oxidase treated cells to examine the distribution of newly synthesized sterols among intracellular ...