After RNA isolation for each sample, double stranded cDNA was synthesized from 500 ng total RNA and biotin-labeled using the GeneChip 3' IVT Express Kit (Affymetrix, Santa Clara, CA, USA, #901228-A) per the manufacturer's instructions and protocol. RNA was purified, quantified, fragmented ran...
Primary antibodies were as follows: anti-CHOP (Sc-575; Santa Cruz Biotechnology, Santa Cruz, CA), anti-Iba1 (WAKO, Richmond, VA), anti-HuC/D clone 16A11 (Invitrogen, Carlsbad, CA), anti-NeuN (Chemicon, Temecula, CA), anti-PLP (Sc18529; Santa Cruz Biotechnology), anti-GFAP (DAKO,...
Primary antibodies against phospho-Akt (Cell Signaling Technology, Beverly, MA, USA), phospho-ERK1/2 (Cell Signaling), phospho-STAT5 (Cell Signaling), phospho-p38 (Cell Signaling Technology), and GAPDH (Chemicon, Temecula, CA, USA) were used at 1:1000 dilution. After several washes, ...
Sections were incubated for 1 h in 10% normal serum with 0.25% Triton X-100 in 20 mM potassium PBS containing 1% BSA (KPBS-BSA), then incubated overnight at 4°C with rabbit polyclonal antiserum to TH (Chemicon, Millipore Temecula, CA; 1:500) or at 4°C with rat monoclonal ...
Rabbit IgG (cat#NI01, Merck Millipore, Temecula, CA, USA) was used as a negative control instead of the primary antibody using the same protein concentration as with the primary antibody. For anti-CD68 staining, the Novolink™ Polymer Detection System kit (cat#RE7140-K, Leica Biosystems, ...
then subjected to 10% or 12% SDS-polyacrylamide gel electrophoresis followed by transfer of separated proteins on 0.45 μm Immobilon-P membrane (Millipore, Bedford, MA, USA). The following primary antibodies were used: mouse monoclonal anti-synaptophysin (1:3000, Millipore, Temecula, CA, USA...
Center of Neurology, Psychiatry, Psychology Via Alfonso Lamarmora 24 50121 Florence, Italy Call: +39 055 587889 Website: Click here to visit— SPAIN Ketamine Clinics —Instituto Dr. Scheib in Palma de Mallorca, SpainFor Depression: $250 / per infusion For Pain: $250 / per infusion The ...
Sections were incubated with either separate primary antibody or a mix- ture of two primary antibodies, for labeling of A1M (chicken anti-human A1M, in-house developed) and Hb (affinity purified goat IgG, GenWay Biotech, San Diego, CA, USA) or PSA-NCAM (Millipore, Temecula, CA, USA),...
(VGluT1, 1:5,000; Chemicon, Temecula, CA, USA) or the antibody to vesicular glutamate transporter 2 (VGluT2, 1:2500; Chemicon, Temecula, CA), in TBS containing 5 % normal donkey serum and 0.05 % Tween-20. After four washes in TBS, 0.05 % Tween-20, sections were incubated for ...
(for PS1-NTF, 1:1000, Chemicon International, Temecula, CA), Ab1997 (for ADAM10, 1:1000, Abcam, Cambridge, MA), EE-17 (for BACE1, 1:1000, Sigma-Aldrich, St. Louis, MO), AF5868 (for Arginase 1, 1:2000, R&D Systems, Minneapolis, MN), sc-6419 (for Clusterin alpha, 1:1000, ...