This analysis is based on the use of effectors (GST–PAK70_106 for Cdc42 and GST-Rhotekin for Rho, Millipore, Temecula, CA, USA) capable of binding activated Cdc42 or Rho. Samples were homogenized in 50 mM Tris (pH 7.4), 1 mM EDTA (pH 8.0), 0.5% Nonidet P-40, 150 mM ...