(1 : 200), Millipore, Cork, Ireland) in PBS/ 0.05% Triton X-100/5% non-fat dried milk, followed by incubation with the following primary antibodies (monoclonal NeuN (1 : 500), Chemicon, Temecula, USA; monoclonal smi-32 (1 : 500), Abcam, Cambridge, UK; monoclonal GFAP (1 : 500)...