In vitro tube formation of HUVECs, Ink4a/ Arf − / − astrocytes, and GSCs was assessed using an in vitro angiogenesis assay kit (Chemicon, Temecula, CA, USA). Control- and Lmo2-Ink4a/Arf −/−astrocytes were cultured for 24 h (5 ×105 cells/10-cm plate) and then used ...
A: White arrowhead indicates spiral ganglion neuron (SGN). B: Black arrows indicate spaces between spiral ganglion neurons. C: White arrowhead indicates spindle-shaped nuclei of Schwann cells. D: Black arrows point to spaces between Schwann cells. Scale bar = 20 μm. Reduced Expression of ...
Western blots were made as previously described18 using the SCIENTIFIC REPORTS | 3 : 1837 | DOI: 10.1038/srep01837 8 www.nature.com/scientificreports following antibodies: a-tubulin mouse IgG (clone B-5-1-2, Sigma-Aldrich); deTyr tubulin rabbit IgG (Chemicon, Temecula, CA); Tyr tubulin...
The story of Rett syndrome: from clinic to neurobiology. Neuron 56: 422–437. Article CAS Google Scholar Chen LY, Rex CS, Babayan AH, Kramar EA, Lynch G, Gall CM et al (2010). Physiological activation of synaptic Rac>PAK (p-21 activated kinase) signaling is defective in a mouse...
Immunohistochemical (IHC) analysis of human pancreatic cancer tissue arrays (PA483a, T143; US Biomax, Rockville, MD, USA) was conducted using monospecific antibodies against MMP-2 (MAB13405; EMD Millipore, Temecula, CA, USA) and TIMP-1 (E3360; Spring Biosciences, Pleasanton, CA, USA) as...
Data ERK1/2, together with either mouse anti-NeuN (1 : 200, were analyzed with a two-way ANOVA followed by Chemicon, Temecula, CA) or mouse anti-S100b (1 : 1000, Bonferroni post hoc correction. Increases in the percent of Sigma, St Louis, MO) in 10% Normal Goat Serum/0.5% ...
Immunohistochemical (IHC) analysis of human pancreatic cancer tissue arrays (PA483a, T143; US Biomax, Rockville, MD, USA) was conducted using mono- specific antibodies against MMP-2 (MAB13405; EMD Millipore, Temecula, CA, USA) and TIMP-1 (E3360; Spring Biosciences, Pleasanton, CA, USA) ...
(Hudson Respiratory Care Inc. [RCI], Temecula, CA) (Fig. 50-4) and the JEM Endotracheal Tube Changer (Instrumentation Industries Inc., Bethel Park, PA). The T.T.X. devices are available in four ODs (2.0-, 3.3-, 4.8-, and 5.8 mm) and two lengths (56 and 81 cm). The smallest...
These data suggest that alteration of ΜΤs is an early event specifically associated with dopaminergic neuron degeneration. Pharmacological stabilization of MTs may be a viable strategy for the management of parkinsonism.Similar content being viewed by others Mitochondrial division inhibitor-1 is ...