The application of PCR to the diagnosis of congenital toxoplasmosis and the comparison with IFA resultsAnimalsMacaca fascicularisTemporal LobeVisual PathwaysNeuronsBrain MappingAnalysis of VarianceColor PerceptionChoice BehaviorReaction TimeWe tested the color selectivity of neurons in the inferior temporal (IT...
There are numerous applications of PCR to aquaculture where the ability to screen large numbers of samples rapidly and at low cost is particularly important. This article contains methods for the preparation of DNA from fish tissues, blood and milt, and its application in PCR reactions. Some ...
investigation journal of veterinary diagnostic leukemia virus importance of primer selection in the application of pcr technology to the diagnosis of bovine G Marsolais,R Dubuc,J Bergeron,... 被引量: 0发表: 0年 Genotyping Of Isolates Of Bovine Leukemia Virus As The Basis Of A Multiplex Test ...
Since last decades, this technique has been widely used in various fi eld of research, especially tumor diagnosis, classifi cation, staging and prognosis diagnosis. This paper outlines the basic principles of real-time PCR technology, and discusses the development and application prospects of the ...
Detection of human cytomegalovirus infection by FQ-PCR technique and its application in the diagnosis and treatment of HCMV infected children 目的建立小儿人巨细胞病毒(HCMV)感染的分子诊断方法.方法采用荧光定量聚合酶链反应(FQ-PCR)法测定45例疑诊HCMV感染的患儿,并与常规PCR及酶联免疫吸附试验(ELISA)比较...
[Objective]To explore the application of Real-time PCR procedure and DNA molecular typing method on dealing with the outbreak of shigellosis.[Methods]Real-time PCR procedure was used to rapidly detect shigellosis in 24 shares enrichment broth of anus mops from people with dysentery,and 5 isolates...
After transfection for 48 h, the DNA of cells was extracted using the QIAamp DNA Mini kit. The primers used for amplification are F: tgtcaaaagccactgtgtcc, R: taaaatctaccaaatcttcacctgt, 200 ng purified polymerase chain reaction (PCR) products containing the sgRNA targets were denatured...
diversity and introduce biases in library composition15. Inhibitors may have even a more detrimental effect during the application of PCR-based methods24. Some specialized ddPCR approaches (e.g. “wild-type” negative, highly multiplexed hotspot or copy number assays29,30,31,32, for example, rely...
PCR detection of drowning related bacterial DNA from organs of drowning victims has attracted huge interest from forensic scientist in recent years. Herein, we established PCR Capillary Electrophoresis (PCR-CE) method for amplifying hemolytic genes in () for freshwater drowning diagnosis. Results were ...
Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop ( Humulus lupulus L.) The cluster analysis of ten hop ( Humulus lupulus L.) varieties and the comparison of amplified DNA polymorphism were assessed using RAPD, STS, ISSR and A... J ...