1991年,期刊“pcr方法与应用”(pcr methods and application)在美国创刊,使有关学者有了自己的论坛和参考的专业期刊。 kary mullis因发明了“聚合酶链式反应”而获得1993年度诺贝尔化学奖。(一名加拿大籍英国科学家michael smith因开创了“寡核苷酸基因定点诱变”的方法而与mullis同享此荣)pcr技术检测细菌 鸭疫里默氏...
These methods, their advantages, and the inconveniences, which are not yet resolved are summarized in the paper. Special focus is given to the possibilities of using these alternative methods for GMO detection in future, when ex 展开 关键词: Alternative methods - DNA amplification - Detection - ...
MethodsandApplication)在美国创刊,使 有关学者有了自己的论坛和参考的专业期 刊。 •KaryMullis因发明了“聚合酶链式反应”而 获得1993年度诺贝尔化学奖。(一名加拿大籍英国 科学家MichaelSmith因开创了“寡核苷酸基因定点诱变”的方法而与 Mullis同享此荣) ...
Based on the standard curve method and other useful data analysis methods, we present and compare four statistical approaches and models for the analysis of real-time PCR data. Results In the first approach, a multiple regression analysis model was developed to derive ΔΔCt from estimation of ...
Rodríguez RA , Pepper IL & Gerba CP ( 2009 ) Application of PCR-based methods to assess the infectivity of enteric viruses in environmental samples . ... Rodríguez,Roberto,A.,... - 《Applied & Environmental Microbiology》 被引量: 212发表: 2009年 Innovation and discovery: the application ...
We aimed to validate the clinical utility of insertion-deletion polymorphisms (INDELs) to confirm presence of a female fetus using cffDNA.Methods: Quantitative real-time PCR (qPCR) for the Y-chromosome-specific sequence, SRY , was performed on cfDNA from 82 samples at 6–39 gestational weeks....
and MR ratio for FeMn spin valve were lost 15 days later in corrosion resistance test under 35°C with 90% humidity conditions MA Innis - 《Pcr Protocols A Guide to Methods & Applications》 被引量: 770发表: 1990年 Les populations sauvages et cultivées de Vitis vinifera L This study pres...
Identification of chicken haplotypes based on the nucleotide sequence of B complex loci could provide more precise identification of haplotypes than traditional serological methods. We report the development and application of polymerase chain reaction with sequence specific primers (PCR-SSP) to type ...
学士学位毕业论文 学号:20084084147 PCR法鉴定小鼠的性别 本科生毕业论文任务书 论文题目PCR法鉴定小鼠的性别 学院名称 指导教师 毕业论文(设计)的内容摘要 应用多重PCR同时扩增雌雄特异基因,使雌雄样品均有扩增产物,本实验针 对小鼠设计了两对引物IL3-1,2和SRY-1,2,通过PCR扩增,扩增产物在1.5%琼 脂糖凝胶上电泳...
Mu D, Yan L, Tang H, Liao Y.A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system.BiotechnolLett. 2015 Jul 18. ...