Real-Time PCR and its Applications in Human Papillomavirus Quantitation and Physical Status IdentificationThe basic principles and advantages of real-time PCR are reviewed in this paper as well as the recent literature regarding to detection and research of human papillomavirus (HPV) in the routine ...
16、 its unique ability to monitor the complete DNA amplification progress and other features such as rapidness ,sensitivity and accuracy.Real-time Quantitative PCR has reformed the conventional PCR as well as enlarge applications.This article reviews the principle and some applications of this new te...
Various derivative technologies based on PCR for nucleic acid detection have emerged with the continuous development and the diverse needs of molecular biology technology. Digital PCR (dPCR) is a nucleic acid detection method for large scale amplification based on a single molecular template, which ru...
"RNA-Seq:Methods and Protocols",作者:Nathan L. St. John、Matthew D. MacManes 和 David W. D. Smith。这篇综述文章介绍了 RNA-Seq 技术的基本原理、技术和应用,包括 RNA-Seq 的基本概念、实验设计、仪器使用和数据分析等方面。 "Reverse transcription and its applications in molecular biology",作者:Ala...
Real-time quantitative PCR and its applications Developed in the mid 1990s for the analysis and quantification of nucleic acid, realtime quantitative PCR is a molecular biological technique gaining rapid... LY Wang,QH Hong,YZ Zhang - 《Chinese Journal of Coll Biology》 被引量: 68发表: 2004年...
In conclusion, since its introduction in the 1980s, PCR continues to prove to be a useful tool with broad applications in discovery biology, medical diagnostics, forensics, and agriculture. References Share For Research Use Only. Not for use ...
例子一2022年10月份，Baccari等人在Environmental Science and Pollution Research上发表了题为“A new TaqMan real-time PCR assay to detect Parachlamydia acanthamoebae and to monitor its co-existence with SARS-COV-2 among COVID-19 patients”的研究性文章。团队在社区获得性肺炎（CAP）的大背景下，通过收集...
2022年10月份,Baccari等人在Environmental Science and Pollution Research上发表了题为“A new TaqMan real-time PCR assay to detect Parachlamydia acanthamoebae and to monitor its co-existence with SARS-COV-2 among COVID-19 patients”的研究性文章。团队在社区获得性肺炎(CAP)的大背景下,通过收集环境水样与...
Tetra-primer Amplification Refractory Mutation System PCR and Its Application in Fauna and Flora Genetics and Breeding Research YU Jun-jian1,2, CHI Mei-li2, JIA Yong-yi2, LIU Shi-li2, ZHU Jun-quan1, GU Zhi-min2 PDF (PC) 1100 摘要/Abstract 摘要: 四引物扩增受阻突变体系PCR(Tetra-...
Once the thermocycler has done its job. Take out your reaction and verify the PCR product with gel electrophoresis. If the PCR is successful, you should see the amplicon at the correct base pair size. And now for some helpful hints when working with PCR. When you are trying to amplify th...