P. Draganov, Z. Kalvatchev, V. Papassavva, S. Spunarova, Real-time PCR and its appli- cations in human papillomavirus quantitation and physical status identification, Biotechnol. Biotechnol. Equip. 18 (2) (2004) 153-160.Giulietti A., Overbergh L., Valckx D., Decallonne B., Bouillon...
In agriculture, PCR plays an integral role in food pathogen detection, plant genotyping for breeding, and GMO testing. In conclusion, since its introduction in the 1980s, PCR continues to prove to be a useful tool with broad applications in ...
Internal amplification control and its applications in PCR detection of foodborne pathogens扩增内标及其在食源性致病菌PCR检测中的应用 Key words: Internal amplification controlPCR detectionfalse-negative results 关键词:扩增内标PCR检测假阴性Despite the improvement of PCR technology, false-... Xiaohua He,Xian...
16、 its unique ability to monitor the complete DNA amplification progress and other features such as rapidness ,sensitivity and accuracy.Real-time Quantitative PCR has reformed the conventional PCR as well as enlarge applications.This article reviews the principle and some applications of this new te...
Here, we present and experimentally validate Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE), a stochastic algorithm for design of multiplex PCR primer sets that minimize primer dimer formation. In a 96-plex PCR primer set (192 primers), the fraction of primer dimers decreases...
Tetra-primer Amplification Refractory Mutation System PCR and Its Application in Fauna and Flora Genetics and Breeding Research YU Jun-jian1,2, CHI Mei-li2, JIA Yong-yi2, LIU Shi-li2, ZHU Jun-quan1, GU Zhi-min2 PDF (PC) 1100 摘要/Abstract 摘要: 四引物扩增受阻突变体系PCR(Tetra-...
Platinum SuperFi II DNA Polymerase amplifies a broad range of sequences with high specificity due to robustness of the enzyme and its specially formulated buffer. The Platinum SuperFi II buffer enables amplification of GC-rich targets without a need for supplementary additives (Figure 10 ...
关键词 : 四引物扩增受阻突变体系 ;单核苷酸多态性(SNP);遗传育种DOI :10.13560/j.cnki.biotech.bull.1985.2019-0575Tetra-primer Amplification Refractory Mutation System PCR and Its Application in Fauna and Flora Genetics and Breeding ResearchYU Jun-jian 1,2 CHI Mei-li 2 JIA Yong-yi 2 LIU Shi-li...
Higher applications in life sciences, such as disease detection, genomics, and cell analysis, further expand its application. Increased investment by the public and private sector and higher awareness increases the application of this device. The escalating prevalence of infectious diseases has prompted ...
BMC Infectious Diseases (2022) 22:375 https://doi.org/10.1186/s12879-022-07356-9 RESEARCH Open Access Development and evaluation of a novel fast broad‑range PCR and sequencing assay (FBR‑PCR/S) using dual priming oligonucleotides targeting the ITS/LSU gene regions for rapid...