we downsampled to specific numbers of reads per cell, and numbers of cells per gRNA. 分析的结果表明the same performance in differential gene expression testing is achieved in TAP-seqat a 19- to 49-fold lowerread depth than that of Perturb-seq。总...
To overcome these limitations, we introduce targeted Perturb-seq (TAP-seq), a sensitive, inexpensive and platform-independent method focusing single-cell RNA-seq coverage on genes of interest, thereby increasing the sensitivity and scale of genetic screens by orders of magnitude. TAP-seq permits ...
Data from n = 621,977 (TAP-seq), n = 67,994 (Perturb-Seq) or n = 637,971 cells (Perturb-seq + gRNA amp.) was used. e, Mean expression per gene for whole transcriptome 10X Genomics compared to TAP-seq, with perturbation target genes highlighted. n = 674 genes ...
Using direct capture Perturb-seq, we also show that targeting individual genes with multiple sgRNAs per cell improves efficacy of CRISPR interference and activation, facilitating the use of compact, highly active CRISPR libraries for single-cell screens. Last, we show that hybridization-based target ...
Figure 4. The depletion of regulatory RNA Pol II subunits perturbs the expression of subsets of genes (A) Principal component analysis of gene expression changes through poly(A) RNA-seq analysis after individual RNA Pol II subunit depletion for 12 h. The dots enclosed in a red circle represen...
Glioblastoma (GBM) is a fatal brain tumour that is traditionally diagnosed based on histological features. Recent molecular profiling studies have reshaped the World Health Organization approach in the classification of central nervous system tumours to
The dynamic exclusion allows acquisition of sufficient structural information to unambiguously confirm the compound's identity without significantly perturbing the quantification obtained with the primary SRM transition list. This prevents the user from wasting precious acquisition time and increases the ...
significant frequency compared to mock-treated controls in all of the genomic sites tested (Fig.6a, Supplementary Fig.11A). To further investigate the safety of our approach, we utilised an unbiased, genome-wide analysis tool and GUIDE-seq (genomewide, unbiased identification of DSBs enabled by ...
ChIP-seq and RNA-seq data generated for this study are available at the Gene Expression Omnibus under accession numberGSE166305. Source data for Figs.1,4–6and Extended Data Figs.1,5and6have been provided as Source Data files. All other data supporting the findings of this study are availab...
Maintaining the proper balance between procoagulant and anticoagulant activity within blood vessels is essential for normal hemostasis (Davie, E. W. et al. (1991)Biochemistry,30(43):10363-10370). Perturbing the balance toward coagulation leads to thrombosis, which can cause heart attack, stroke, ...