Genetic screens help infer gene function in mammalian cells, but it has remained difficult to assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop Perturb-seq, combining single-cell RNA sequencing (RNA-seq) and clustered regularly interspaced short palindromic repeats ...
IN VIVO PERTURB-SEQ: STUDY GENE FUNCTION AT SCALE IN DEVELOPING TISSUESdoi:10.1016/j.euroneuro.2021.07.070Xin JinElsevier BVEuropean Neuropsychopharmacology