Thetranscriptomecontains rich information on molecular, cellular, and organismal phenotypes. However, experimental and statistical limitations constrain sensitivity and throughput ofgenetic screening with single-cell transcriptomics readout. To overcome these limitations, we introducetargeted Perturb-seq (TAP-seq...
To overcome these limitations, we introduce targeted Perturb-seq (TAP-seq), a sensitive, inexpensive and platform-independent method focusing single-cell RNA-seq coverage on genes of interest, thereby increasing the sensitivity and scale of genetic screens by orders of magnitude. TAP-seq permits ...
Data from n = 621,977 (TAP-seq), n = 67,994 (Perturb-Seq) or n = 637,971 cells (Perturb-seq + gRNA amp.) was used. e, Mean expression per gene for whole transcriptome 10X Genomics compared to TAP-seq, with perturbation target genes highlighted. n = 674 genes ...
Direct-capture Perturb-seq enables detection of multiple distinct sgRNA sequences from individual cells and thus allows pooled single-cell CRISPR screens to be easily paired with combinatorial perturbation libraries that contain dual-guide expression vectors. We demonstrate the utility of this approach ...
Figure 4. The depletion of regulatory RNA Pol II subunits perturbs the expression of subsets of genes (A) Principal component analysis of gene expression changes through poly(A) RNA-seq analysis after individual RNA Pol II subunit depletion for 12 h. The dots enclosed in a red circle represen...
Glioblastoma (GBM) is a fatal brain tumour that is traditionally diagnosed based on histological features. Recent molecular profiling studies have reshaped the World Health Organization approach in the classification of central nervous system tumours to
The dynamic exclusion allows acquisition of sufficient structural information to unambiguously confirm the compound's identity without significantly perturbing the quantification obtained with the primary SRM transition list. This prevents the user from wasting precious acquisition time and increases the ...
(Fig.1e), and their levels were much lower than the TP53 mRNA level encoded by the introduced plasmid. Further, because concentrations of the affected microRNAs were also much lower than the siKRASG12Dlevel, it is unlikely any individual mRNA or microRNA in the EVs will perturb the ...
ChIP-seq and RNA-seq data generated for this study are available at the Gene Expression Omnibus under accession numberGSE166305. Source data for Figs.1,4–6and Extended Data Figs.1,5and6have been provided as Source Data files. All other data supporting the findings of this study are availab...
Maintaining the proper balance between procoagulant and anticoagulant activity within blood vessels is essential for normal hemostasis (Davie, E. W. et al. (1991)Biochemistry,30(43):10363-10370). Perturbing the balance toward coagulation leads to thrombosis, which can cause heart attack, stroke, ...