To try to understand this difference and to confirm that the II1 transgene was inserted at the expected position, we performed long-read sequencing with the Oxford Nanopore MinION in conjunction with the nCATS protocol29to enrich for sequencing reads covering the region around the insertion site (...
et al. Targeted massively parallel sequencing of autism spectrum disorder-associated genes in a case control cohort reveals rare loss-of-function risk variants. Mol. Autism 6, 43 (2015). Article CAS PubMed PubMed Central Google Scholar Rhodes, C.T. et al. Cross-species analyses unravel the...
Thus, there is need for a new sequencing approach that is both rapid and cost-effective, yet offers robust assemblies suitable for genome-editing applications. Linked read technology (or read clouds) has the potential to fill this application gap by combining aspects of short and long reads via...
The whole RNA next-generation sequencing reads were aligned to the mouse reference genome with STAR (version 2.7.8). The HTseq (version 0.13.5) tool was used to perform read summarization. The R package DESeq2 (v1.30.0) was used to conduct differential expressed gene (DEG) analysis. Prin...
(E) Sequencing of the y1a mutant shows a single point deletion compared to wild-type Oregon R flies at the y1 cleavage site. Target sites are indicated in orange, the protospacer adjacent motif (PAM) in red, and the cleavage site by a black triangle. (F) HRMA shows a reproducible shift...
In the future, scientific advances may eventually allow scientists to combine mutagenesis screens through next generation sequencing and proteomic techniques with the computational modeling of compound interactions with all possible mutant variants of a targeted kinase. This will lead to the development of...
Comparative genomics approaches such as resistance gene enrichment sequencing (RenSeq) can also be employed to rapidly identify genomic variants in defence-related genes that are linked to disease phenotypes (Jupe et al. 2014). (ii) Field tests are necessary for the evaluation of agronomic fitness,...
Deep intronic mutation in OFD1, identified by targeted genomic next-generation sequencing, causes a severe form of X-linked retinitis pigmentosa (RP23). X-linked retinitis pigmentosa (XLRP) is genetically heterogeneous with two causative genes identified, RPGR and RP2. We previously mapped a locus...
Chromatin immunoprecipitation sequencing (ChIP-seq) HCT116 cells were treated either with vehicle, PDD (10 μM, 4 h), or MZ1 (30 nM, 1 h). Chromatin was cross-linked with 1% formaldehyde at room temperature for 10 min and sonicated in SDS-lysis buffer (1% SDS, 50 mM Tr...
Middle: sequencing of gDNA from wild type NALM-6 cells, in which the TdT sequence corresponds to the sequence deposited in the database. Bottom: after cloning of CRISPR/Cas9-modified NALM-6 cultures, the selected clone shows a clean deletion in both alleles of the TdT gene, including the ...