Thus, there is need for a new sequencing approach that is both rapid and cost-effective, yet offers robust assemblies suitable for genome-editing applications. Linked read technology (or read clouds) has the potential to fill this application gap by combining aspects of short and long reads via...
In this work, by combining the CRISPR-based enrichment with 10x Genomics linked-read sequencing and the PacBio HiFi long-read platform (Fig.1a), we were able to achieve targeted haplotype-resolved assembly of the MHC region from diploid human cells. We also applied our targeted approach to tw...
To try to understand this difference and to confirm that the II1 transgene was inserted at the expected position, we performed long-read sequencing with the Oxford Nanopore MinION in conjunction with the nCATS protocol29to enrich for sequencing reads covering the region around the insertion site (...
Sequencing quality control consisted of base quality score recalibration across flow cells and instruments as well as removal of duplicate reads resulting from PCR library amplification bias. For high-confidence genotype calling, we selected variants with a read depth greater than 8X, a call rate of...
Circulating tumor DNA ELISA: Enzyme-linked immunosorbent assay FNA: Fine needle aspiration gDNA: Genomic DNA NSG: Next-generation sequencing PBL: Peripheral blood leukocyte SD: Standard deviation SNP: Single nucleotide polymorphism SNV: Single nucleotide variant ...
TNF-α tumor necrosis factor-alpha, Foxp3 forkhead box protein 3.cTumor occurrence and progression are closely linked to TME. Overactivation of NF-κB signaling not only promotes tumor cell survival, invasion, metastasis, genomic instability, and metabolic abnormalities, but also reshapes the immune...
Further information on research design is available in theNature Research Reporting Summarylinked to this article. Data availability The data supporting the findings from this study are included either in the manuscript or its associated supplementary files. Sequencing data has been deposited to the Sequ...
Wiskott-Aldrich syndrome (WAS) is an X-linked primary immunodeficiency with severe platelet abnormalities and complex immunodeficiency. Although clinical gene therapy approaches using lentiviral vectors have produced encouraging results, full immune and
However, caffeine itself has been not reported in grasses while at least some of the matched metabolites can be linked with purine salvage or degradation [45]. Histidine and vitamin B6 metabolism had a shared effect in leaves and spikes. Besides protein synthesis, histidine is tightly connected ...
As the use of next-generation sequencing in clinical practice becomes more frequent, oncologists often find BRAFnon-V600 mutations in their patients’ malignancies, but they are unsure of feasible therapeutic alternatives for optimum therapy. Dabrafenib or vemurafenib, which are single-agent BRAF inhibi...