To try to understand this difference and to confirm that the II1 transgene was inserted at the expected position, we performed long-read sequencing with the Oxford Nanopore MinION in conjunction with the nCATS protocol29to enrich for sequencing reads covering the region around the insertion site (...
et al. Targeted massively parallel sequencing of autism spectrum disorder-associated genes in a case control cohort reveals rare loss-of-function risk variants. Mol. Autism 6, 43 (2015). Article CAS PubMed PubMed Central Google Scholar Rhodes, C.T. et al. Cross-species analyses unravel the...
Thus, there is need for a new sequencing approach that is both rapid and cost-effective, yet offers robust assemblies suitable for genome-editing applications. Linked read technology (or read clouds) has the potential to fill this application gap by combining aspects of short and long reads via...
In a paired-end experiment the target region contained both ends of the circularized fragments, while single-read sequencing targeted only 3' ends of the circularized fragments. To assess the specificity of the capture we compared the numbers of sequence reads mapping inside and outside the target...
Comparative genomics approaches such as resistance gene enrichment sequencing (RenSeq) can also be employed to rapidly identify genomic variants in defence-related genes that are linked to disease phenotypes (Jupe et al. 2014). (ii) Field tests are necessary for the evaluation of agronomic fitness,...
The whole RNA next-generation sequencing reads were aligned to the mouse reference genome with STAR (version 2.7.8). The HTseq (version 0.13.5) tool was used to perform read summarization. The R package DESeq2 (v1.30.0) was used to conduct differential expressed gene (DEG) analysis. Prin...
In the future, scientific advances may eventually allow scientists to combine mutagenesis screens through next generation sequencing and proteomic techniques with the computational modeling of compound interactions with all possible mutant variants of a targeted kinase. This will lead to the development of...
(F) Sequencing of induced mutations in the white gene, as in (E). We tested a variety of concentrations of Cas9 mRNA:sgRNA and found the efficiency of NHEJ-based deletions to be concentration dependent (Table 1). Remarkably, at the highest concentration (1,000 ng/μl), we found that ...
M. et al. Targeted sequencing of refractory myeloma reveals a high incidence of mutations in CRBN and Ras pathway genes. Blood 128, 1226–1233 (2016). Article CAS PubMed PubMed Central Google Scholar Kurimchak, A. M. et al. The drug efflux pump MDR1 promotes intrinsic and acquired ...
The targeting vector was confirmed by restriction enzyme analysis and sequencing after each modification. The boundaries of the pCAG-stop cassette and BGHpA-ROSA26 genomic sequences were confirmed by sequencing with primers ROSASQ1 and ROSASQ2. In order to test the efficacy for labeling of an ...