In this study, we explored how the long-range sequence information of barcode linked-read sequencing (BLRS) can be leveraged to improve repeat-read detection. We also devised a novel algorithm using BLRS barcodes for distance estimation and evaluated its application for STR genotyping. Both ...
the heterozygosity of the organism and the sequencing coverage are two parameters that influence the sensitivity of ReMIX to detect true positive recombinants. To evaluate the sensitivity, we performed
2017. Linked read sequencing resolves complex genomic rearrangements in gastric cancer metastases. Genome Med 9: 57. http://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0447-8.Greer,S.U., Nadauld,L.D., Lau,B.T., Chen,J., Wood-Bouwens,C., Ford,J.M., Kuo,C.J. and ...
Though massively parallel sequencing has been widely applied to noninvasive prenatal screen for common trisomy, the clinical use of massively parallel sequencing to noninvasive prenatal diagnose monogenic disorders is limited. This study was to develop a method for directly determining paternal haplotypes ...
Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) is a simple and scalable NGS library technology that generates barcode linked-reads for genome scale sequencing applications. The whole procedure can be carried out in a PCR tube without the need for expensive instrumentation. The TELL-Se...
Linker is a suite of C++ tools useful for interpreting long and linked read sequencing of cancer genomes. The most significant information long read sequencing provides is the local haplotype of a sample. In cancer cell lines where Aneuplpoidy, Loss of Heterozygosity, and Structural Variation is...
To address this challenge, we performed AR-targeted linked-read DNA-sequencing of CRPC cell line and patient derived xenograft (PDX) models with the goal of resolving sub-clonal and complex AR SVs. This approach confirmed the heterogeneity of AR SVs in these PDXs, including phased AR SVs ...
Linked-read sequencing provides long-range information on short-read sequencing data by barcoding reads originating from the same DNA molecule, and can improve detection and breakpoint identification for structural variants (SVs). Here we present LinkedSV for SV detection on linked-read sequencing data...
28Branches19Tags Code README GPL-3.0 license ARCS Scaffolding genome sequence assemblies using linked or long read sequencing data Contents Description ARCS and ARKS are genome sequence assembly scaffolders using linked and long read sequencing data ...
The recently developed linked-read sequencing technology from 10X Genomics combines a novel bar-coding strategy with Illumina sequencing. This technology labels all reads that originate from a small number (similar to 5 to 10) DNA molecules similar to 50 Kbp in length with the same molecular ...