1. Dilute the DNA sample 1:5 (Dilute more or less depending on DNA concentration.) 2. Add 5µL DNA, and 5µL AD primers to PCR plate according to the diagram below (each AD primer has a specific concentration, seeAdditional Informationat the end of the protocol): NOTE: Keep plat...
25. The PCR products must be purified before they can be squenced. This can be done individually via the Qiagen PCR purification protocol or enzyme purified as explained in this protocol. Transfer 5µ: of 2° reaction PCR products to a new plate. (Again, this is very easy with a multi...
Fig. 1 The protocol of thermal asymmetric interlaced PCR TAIL-PCR 一般分为3 次反应(图1) 。第1 次PCR 反应由5 次高特异性反应、1 次低特异性反应、10 次较低特异性反应和12 次热不对称的超级循环构成。通过5 次高特异性的反应,使sp1 与已知的序列(载体或T2DNA 等) 退火并延伸,提高目标序列的浓度。
分析测试,百科网,TAILPCR(thermalasymmetricinterlacedPCR)简介, 分子生物学研究中,基因克隆和分子杂交的探针制备等操作常需分离与已知DNA序列邻近的未知序列,TAIL-PCR又叫热不对称交错PCR,能够较好地解决上述难题。该技术通过3个嵌套的特异性引物分别和简并引物组合进行
内容提示: 一种DNA侧翼序列分离技术TAIL—PCR罗丽娟112,施季森“t1南京林业大学,江苏 南京 210037;2.华南热带农业大学,海南 儋州571757)摘要:在分子生物学研究中,基因克隆和分子杂交的探针制备等操作常需分离与已知DNA序列邻近的未知序列,热不对称交错PCR(简称‘FAII,一PCR)反应技术能够较好地解决上述难题。该技术通过...
1 The protocol of thermal asymmetric interlaced PCRTAIL-PCR 一般分为3 次反应(图1 。第1 次PCR 反应由5 次高特异性反应、1 次低特异性反应、10 次较低特异性反应和 10、12 次热不对称的超级循环构成。通过5 次高特异性的反应,使sp1 与已知的序列(载体或T2DNA 等 退火并延伸,提高目标序列的浓度。1 ...
TAIL-PCRT-DNAinsertionalmutant flanking sequence isolationprotocol TAIL–PCR Primary PCR Reaction system (20μL): Rice DNA 40ng; 10ⅹPCR buffer 2μL 150μMdNTPs 1.5mMMgCl2 0.15μM specific primer 1μM AD primer 0.5unitTaqDNApolymerase(Takara公司) Secondar Reaction system (20μL): DNA ...
the same PCR mixture and the same PCR condition.All the TAIL PCR reaction need only a few time as in one day.It has become a powerful tool in a genomic analysis.This article presents the principle and protocol of TAIL PCR.Methods for primers design and products selection are also ...
Based on this protocol, HT-TAIL-PCR may be easily adapted for other organisms.doi:10.1385/1-59259-413-1:241Tatjana SingerEllen BurkeHumana PressMethods in Molecular BiologySinger, T., and Burke, E. (2003). High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions. In Plant ...
pcrtail序列dna罗罗侧翼 Tail-PCR:种一DNA种种翼序列分离技罗罗娟1,2,施季森13(1.罗罗罗南京林大学,罗罗罗罗罗江 南京 210037;2.罗罗罗罗罗罗罗南大学,海南 儋州 571737)摘要:在分子生物学研究中,基因克隆罗罗罗罗罗罗罗罗罗罗罗罗罗罗罗罗罗和分子交的探制等操作常需分离与已知DNA罗罗罗罗罗罗罗序列近的未知序...