doi:10.1007/978-1-62703-739-6_22Sai-MeiHouMolecular Toxicology ProtocolsHou SM. The human T-cell cloning assay: Identifying genotypes susceptible to drug toxicity and somatic mutation. Methods Mol Biol. 2014;1105:283-301.
In order to establish target cells for bioluminescence-based cytotoxicity assay, a panel of cell lines were generated via lentiviral transduction including SW620-luciferase, SW620-HLA-A11-luciferase, CFPAC1-luciferase, CFPAC1-HLA-A11-luciferase, PANC-1-luciferase, and PANC-1-KRAS-G12V-luciferase....
In spite of the success of CAR T-cell therapies in B-cell malignancies, there are currently few options for the 40–50% of patients that relapse2,5, and B-ALL patients >25 years are currently ineligible for commercially available CAR 19 T cell therapy due to toxicity concerns. Patient...
NK Cell Depletion Assay Untransduced T cells or T cells expressing either IC9-Luc90-CD828Z or Hu19-CD828Z were cultured with autologous PBMCs in a 1:1 ratio. The cocultures were treated with either 10 ng/mL of AP1903 or vehicle control. 24 h after the cultures were set up, the ...
Yee C, Thompson JA, Byrd D, Riddell SR, Roche P, Celis E: Adoptive T cell therapy using antigen-specific CD8+ T cell clones for the treatment of patients with metastatic melanoma: in vivo persistence, migration, and antitumor effect of transferred T cells. Proc Natl Acad Sci USA. 2002,...
Single-cell sequencing technologies for CAR T-cells Single-cell sequencing technologies used in CAR T-cell research mainly include scRNA-seq, single-cell T-cell receptor sequencing (scTCR-seq), single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq), cytometry by time-of-flig...
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Chimeric antigen receptor (CAR) T cells used for the treatment of B cell malignancies can identify T cell subsets with superior clinical activity. Here, using infusion products of individuals with large B cell lymphoma, we integrated functional profiling using timelapse imaging microscopy in nanowell...
The ability to image adoptively transferred T cells in the body and to eliminate them to avoid toxicity will be vital for chimeric antigen receptor (CAR) T cell therapy, particularly against solid tumors with higher risk of off-tumor toxicity. Previously, we have demonstrated the utility of som...
In recent years, adoptive cell therapy has gained a new perspective of application due to the development of technologies and the successful clinical use of CAR-T cells for the treatment of patients with malignant B-cell neoplasms. However, the efficacy of CAR-T therapy against solid tumor remai...