Fluorescent target array killing assay: a multiplex cytotoxic T-cell assay to measure detailed T-cell antigen specificity and avidity in vivo. Cytometry. Part A 81 (8), 679.Quah BJ, Wijesundara DK, Ranasinghe C et al. Fluorescent target array: a multiplex cytotoxic T-cell assay to ...
Tools to study CD8+T cell subsets References Figure 1. Cytotoxic T cell (CD8+T cell) engaging with target cell for destruction. Development of CD8+T cells The development of CD8+T-αβ cells begins with lymphoid progenitors in the thymus, which proceed through...
We next sought to characterize changes in tumor and oHSV specific CD8+ T-cell responses following oHSV injection into tumors in brain. Since neither GL261 nor CT2A tumor antigens have been well characterized, we needed to utilize GBM cells, engineered to express the GP33 (gp33-41), a mo...
In vivo OVA-specific Cytotoxic CD8+ T Cell Killing Assay Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived from infection with a p... N Chaoul,C Fayolle,C Leclerc - BIO-PROTOCOL 被引量: 0发表: 2016年 in ...
A non-specific cytotoxic cell assay for fish is presented that is based on the release of the activated fluorochrome calcein AM from lysed carp epithelioma papulosum cyprini (EPC) cells. To establish the suitability of treating EPC cells with calcein AM the uptake and spontaneous release of th...
a process where virus-specific CD8+T cells have poor cytokine output, cytolytic activity, and impaired proliferative capacity are actively inhibited7,8. Disruption in CD8+T cell exhaustion can lead to severe and even fatal T cell immunopathology after viral infection7,8whereas its presence can impr...
An object of the present invention is to provide a cytotoxic assay using a new established fish cell line. The invention attained according to the object is a cytotoxic assay, wherein an evaluation of toxicity of a specimen is performed on the basis of its toxicity to an established cell lin...
The cell culture and MTT assay were performed using the method described previously [35,36]. 5. Conclusions Four new C17 polyacetylenes, ginsenoyne O (1), ginsenoyne P (2), ginsenoyne Q (3), and 3-acetyl panaxytriol (4), along with fifteen known ones (5-19), were isolated from ...
The invention provides cytotoxic T-ALL cell lines, and modified cytotoxic T-ALL cell lines which contain a heterologous DNA sequence. The DNA sequence may encode a product capable of stabilizing or po
Disulfiram/copper-induced apoptosis in GBM cell lines. (A) Annexin V assay. The GBM cells were exposed to 0.5 μ M of disulfiram (DS), 1 μ M of CuCl2 (Cu), 1 μ M of gemcitabine (dFdC) or disulfiram plus CuCl2 (DS/Cu) and disulfiram plus CuCl2 plus gemcitabine (DS/Cu/...