In the current chapter, the increased adhesiveness of CTL, following specific peptide/MHC class I recognition, forms the basis of a simple, peptide-specific assay, the cytotoxic T-cell adherence assay (CAA) (12), which correlates well with more traditional effector assays, such as 51Cr release...
Tools to study CD8+T cell subsets References Figure 1. Cytotoxic T cell (CD8+T cell) engaging with target cell for destruction. Development of CD8+T cells The development of CD8+T-αβ cells begins with lymphoid progenitors in the thymus, which proceed through ...
We next sought to characterize changes in tumor and oHSV specific CD8+ T-cell responses following oHSV injection into tumors in brain. Since neither GL261 nor CT2A tumor antigens have been well characterized, we needed to utilize GBM cells, engineered to express the GP33 (gp33-41), a mo...
a process where virus-specific CD8+T cells have poor cytokine output, cytolytic activity, and impaired proliferative capacity are actively inhibited7,8. Disruption in CD8+T cell exhaustion can lead to severe and even fatal T cell immunopathology after viral infection7,8whereas its presence can impr...
A non-specific cytotoxic cell assay for fish is presented that is based on the release of the activated fluorochrome calcein AM from lysed carp epithelioma papulosum cyprini (EPC) cells. To establish the suitability of treating EPC cells with calcein AM the uptake and spontaneous release of th...
In vivo OVA-specific Cytotoxic CD8+ T Cell Killing Assay Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived from infection with a p... N Chaoul,C Fayolle,C Leclerc - BIO-PROTOCOL 被引量: 0发表: 2016年 In ...
To determine the effect of these TGF-β-blocking strategies on T cell-mediated tumor lysis, CD8+ T cells were assessed for cytotoxic activity against EL4 tumor cells, using a standard 51Cr-release assay (Heusel et al., 1994). When cultured with labeled EL4 tumor cells directly following puri...
This invention concerns an assay for cytotoxic T cells. BACKGROUND OF THE INVENTION Cytotoxic T cells (or cytotoxic T lymphocytes (CTL), as they are also known) are one of the types of cells that develop from lymphoid stem cells and that play an important role in the immune system. T ce...
The invention provides cytotoxic T-ALL cell lines, and modified cytotoxic T-ALL cell lines which contain a heterologous DNA sequence. The DNA sequence may encode a product capable of stabilizing or po
The cytotoxic activity of curcumin towards CCRF-CEM human T-cell leukemia cells was measured by the MTT assay. Tumor cells were more sensitive to the cytotoxic activity of curcumin or curcumin-Cu (II)compared to normal cells, and the IC50 ofcurcumin towards CCRF-CEM cells was 8.68 µM, an...