Little to no cytotoxicity was observed in RAW264.7 murine macrophages, Jurkat T lymphocytes,RAJI B lymphocytes, and WHTBF-6 lung fibroblasts after 24 hour treatment with up to 2.5 µg/cm2 nanoparticles. Theab
CD8+ tumor-infiltrating lymphocytes (TILs) mediate tumor rejection through recognition of tumor antigens and direct killing of transformed cells. Effector CD8+ T cells in the tumor microenvironment produce IL-2, IL-12, and IFNγ, which promote the cytotoxic ability ...
1.2.2.3.2Cytotoxic T-cells Cytotoxic T-cells, also referred to as CD8+T-cells because they express a CD8 glycoprotein on theirsurface, are the mediators of CMC, killing host cells infected with intracellular pathogens and tumor cells, usingMHC class Irestriction as explained inSection 1.2.2.1....
Tumor single-cell suspensions were enriched for immune cells and all single-cell transcriptomes annotated to cell states (see STAR Methods). We detected three main cell clusters containing T and natural killer (NK) cell, myeloid cell, and non-immune cell states, as well as three minor clusters...
produce IL-2, IL-12, and IFNγ, which promote the cytotoxic ability of CD8+T cells, leading to targeting of tumor cells for killing. Elevated levels of cytotoxic CD8+T cells in the tumor microenvironment are correlated with improved anti-tumor effects and progno...
Mechanical force contributes to perforin pore formation at immune synapses, thus facilitating the cytotoxic T lymphocytes (CTL)-mediated killing of tumor cells in a unidirectional fashion. How such mechanical cues affect CTL evasion of perforin-mediated
To formally test whether inhibition was due to induction of immunosuppressive CD4+T cells, we established an adoptive T cell transfer (ACT) system inRag2−/−mice (see Methods). ACT of Tregcell-depleted CD4+/CD8+T cells from LDVax-treated WT mice into T3 tumour-bearing, immunodeficient...
Although cytotoxic CD8+ T lymphocytes (CTLs) are essential for anti-tumour immunity, they are frequently dysfunctional in tumours1. Cytokines that sustain CTL activity are attractive for cancer immunotherapy, but avoiding inflammatory toxicity remains a
Because agents capable of killing tumour cells (cytotoxic drugs) are likely the most promising in the clinic, there is a need for drug sensitivity assays that reliably identify cytotoxic compounds that induce cell death. We recently developed a drug sensitivity assay, called the RNA disruption ...
The killing of the fluorescently labeled target B cells (see NK cell section) is used to estimate cytotoxic activity. Antigen-specific effector cells are quantified in the total CD8 T-cell pool using MHC-peptide tetramers.14 A tetramer-based approach to quantifying and measuring the function of...