InnoModels Biotechnology 的 T 细胞介导的杀伤试验是一种具有巨大潜力的抗癌治疗方法。通过利用患者自身的T细胞,该检测能够有效杀死肿瘤细胞,为患者提供新的治疗选择。InnoModels Biotechnology将继续努力促进该检测在临床应用中的进一步发展。
After 60 years of intense fundamental research into T cell-mediated cytotoxicity, we have gained a detailed knowledge of the cells involved, specific recognition mechanisms and post-recognition perforin–granzyme-based and FAS-based molecular mechanisms.
We conducted a surfaceome-focused CRISPRa screen of K562 cells to identify tumor ligands that modulate NK cell-mediated killing. K562 cells are a NK-sensitive chronic myelogenous leukemia cell line that is deficient in MHC class I expression. For this screening process, we generated a clonal iso...
Since LT-α does not directly induce killing of D5 tumor cells in vitro, but does stimulate D5 tumor cells to secrete chemokines, these data suggest a model where LT-α expression by tumor-specific effector T cells interacts via cross-linking of the LT-βR on tumor cells to induce ...
Impedance-based tumor cell killing assay (xCELLigence) The xCELLigence (ACEA, San Diego, CA) long-term tumor cell killing was used over 100–160 h. Tumor cells were expanded for 18–24 h before T-cells were added. The cell index was monitored every 15-min. A decreasing cell index ...
anti-PD-L1 single-cell sequencing cytotoxic CD4+ T cells predictive gene signature Introduction Immunotherapies have changed the landscape of cancer treatment by producing durable and long-lasting responses through triggering anti-tumor cell-mediated immunity. In particular, checkpoint inhibitors (CPIs) tar...
Chimeric antigen receptor (CAR) T cell (CAR-T cell) therapy based on gene editing technology represents a significant breakthrough in personalized immunotherapy for human cancer. This strategy uses genetic modification to enable T cells to target tumor-s
(Supplementary Fig.10a)27, to treatArih1-WT-OE cells. Similar toSting-knockdown, we observed that treatment with C-176 significantly abrogated the anti-tumor effects of PD-L1 blockade therapy inArih1-WT-OE tumors, together with decreased CD8+and GzmB+CD8+T cell infiltration, and reduced ...
SERPINB6B prevents gasdermin D-mediated induction of pyroptosis. In this way, SERPINB6B suppresses immunogenic cell death and confers evasion of T cell-mediated tumor lysis of incipient metastatic cells. On-target inhibition of Malat1 using therapeutic antisense nucleotides suppresses metastasis in a ...
Chimeric antigen receptor (CAR) T cell is a novel approach, which utilizes anti-tumor immunity for cancer treatment. As compared to the traditional cell-mediated immunity, CAR-T possesses the improved specificity of tumor antigens and independent cytotoxicity from major histocompatibility complex molecul...