T细胞杀伤实验测试(T cell Killing Assay) 东大生物的T细胞杀伤测定法可鉴定受测品在促进或抑制T细胞活性后对靶肿瘤细胞杀伤/变态反应作用的影响程度。 这一方法可用于抗体受测品,如双特异性抗体(bscAb),特别是双特异性T细胞衔接子(BiTE)子类的抗体。 最终,将通过流式细胞术(FCM)通过活/死染料检查靶肿瘤细胞的生...
In vitro T cell killing assay Purified OT-1 T cells were activated by anti-CD3 and anti-CD28 for 48 h. Purified DCs were loaded with 5 μg/ml OT-1 peptides for 30 min at 37 °C. After washed, DCs were coincubated with activated OT-1 T cells for 4 h at an E:T rat...
InnoModels Biotechnology 的 T 细胞介导的杀伤试验是一种具有巨大潜力的抗癌治疗方法。通过利用患者自身的T细胞,该检测能够有效杀死肿瘤细胞,为患者提供新的治疗选择。InnoModels Biotechnology将继续努力促进该检测在临床应用中的进一步发展。
This invention regards the T cell response for the test antigen in regard to new T cell assay method, especially, with the removal of the controllable T cell rising. In order to make the detection of T cell response optimize, with the antigen or other sample the time when it cultures (...
In vivo OVA-specific Cytotoxic CD8+ T Cell Killing Assay Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived from infection with a p... N Chaoul,C Fayolle,C Leclerc - BIO-PROTOCOL 被引量: 0发表: 2016年 in ...
Cytotoxic T-cell-killing assay of liquid tumour cells Preparation of target cells Target cells (for example, NALM-6) were fluorescently stained using CellTrace CSFE Cell Proliferation Kit (Thermo Fisher,C34554) according to the manufacturer’s protocol. ...
今天分享一个测试T细胞增殖的实验,可用于测试一些蛋白对于T细胞增殖的影响。 CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell ...
1.酶联板(Assay plate):一块(96孔)。 2.标准品(Standard):2瓶(冻干品)。 3.样品稀释液(Sample Diluent):1×20ml/瓶。 4.生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。 5.辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。
英文全称:Inducible T-Cell Co Stimulator Ligand 检测范围:0.25ng/ml~8ng/ml 灵敏度:0.1 货号: FS15675 试剂准备: 1. 使用前将所有的试剂和标本缓慢均衡至室温(18-25oC),试剂不能直接在37oC溶解。 2. 标准品(冻干品):每瓶标准品加入标准品稀释液1mL,盖好后室温静置大约10分钟,同时反复颠倒/搓动以助溶解...
原代T细胞或PBMC及Jurkat T 细胞激活测试(T Cell Activation Assay) T细胞或PBMC活化测定试验确定受试品对T细胞或PBMC在细胞活化过程中的调节作用。 东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞或PBMC 活化中的作用,测试方式包括用细胞因子的ELISA或流式细胞仪(FCM)的测定。